Formalin-Fixed Paraffin-Embedded Tissues

Methods and Protocols

Editors:

ISBN: 978-1-61779-054-6 (Print) 978-1-61779-055-3 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii

  2. No Access

    Book Protocol

    Pages 1-21

    Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Research

  3. No Access

    Book Protocol

    Pages 23-35

    Tissue Microarrays: Construction and Uses

  4. No Access

    Book Protocol

    Pages 37-68

    Standardization in Immunohistology

  5. No Access

    Book Protocol

    Pages 69-77

    Multiple Immunofluorescence Labeling of Formalin-Fixed Paraffin-Embedded Tissue

  6. No Access

    Book Protocol

    Pages 79-89

    Microwaves for Chromogenic In Situ Hybridization

  7. No Access

    Book Protocol

    Pages 91-103

    Automated Analysis of FISH-Stained HER2/neu Samples with Metafer

  8. No Access

    Book Protocol

    Pages 105-115

    Laser Capture Microdissection of FFPE Tissue Sections Bridging the Gap Between Microscopy and Molecular Analysis

  9. No Access

    Book Protocol

    Pages 117-129

    Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections

  10. No Access

    Book Protocol

    Pages 131-145

    Microarray-Based CGH and Copy Number Analysis of FFPE Samples

  11. No Access

    Book Protocol

    Pages 147-160

    Microarray Profiling of DNA Extracted from FFPE Tissues Using SNP 6.0 Affymetrix Platform

  12. No Access

    Book Protocol

    Pages 161-180

    Whole Genome Amplification of DNA Extracted from FFPE Tissues

  13. No Access

    Book Protocol

    Pages 181-190

    Pyrosequencing of DNA Extracted from Formalin-Fixed Paraffin-Embedded Tissue

  14. No Access

    Book Protocol

    Pages 191-204

    Analysis of DNA Methylation in FFPE Tissues Using the MethyLight Technology

  15. No Access

    Book Protocol

    Pages 205-237

    RT-PCR Gene Expression Profiling of RNA from Paraffin-Embedded Tissues Prepared Using a Range of Different Fixatives and Conditions

  16. No Access

    Book Protocol

    Pages 239-257

    RT-PCR-Based Gene Expression Profiling for Cancer Biomarker Discovery from Fixed, Paraffin-Embedded Tissues

  17. No Access

    Book Protocol

    Pages 259-267

    MicroRNA Isolation from Formalin-Fixed, Paraffin-Embedded Tissues

  18. No Access

    Book Protocol

    Pages 269-280

    Gene Expression Profiling of RNA Extracted from FFPE Tissues: NuGEN Technologies’ Whole-Transcriptome Amplification System

  19. No Access

    Book Protocol

    Pages 281-295

    Protein Mass Spectrometry Applications on FFPE Tissue Sections

  20. No Access

    Book Protocol

    Pages 297-307

    An Alternative Fixative to Formalin Fixation for Molecular Applications: The RCL2®-CS100 Approach

  21. Back Matter

    Pages 309-312