Protein Microarray for Disease Analysis

Methods and Protocols

Editors:

ISBN: 978-1-61779-042-3 (Print) 978-1-61779-043-0 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xiii

  2. Protein-Detecting Analytical Microarrays

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-13

      Detecting and Quantifying Multiple Proteins in Clinical Samples in High-Throughput Using Antibody Microarrays

    3. No Access

      Book Protocol

      Pages 15-28

      Analysis of Serum Protein Glycosylation with Antibody–Lectin Microarray for High-Throughput Biomarker Screening

    4. No Access

      Book Protocol

      Pages 29-36

      Antibody Suspension Bead Arrays

    5. No Access

      Book Protocol

      Pages 37-55

      Reverse Protein Arrays Applied to Host–Pathogen Interaction Studies

    6. No Access

      Book Protocol

      Pages 57-66

      Identification and Optimization of DNA Aptamer Binding Regions Using DNA Microarrays

    7. No Access

      Book Protocol

      Pages 67-77

      Recombinant Lectin Microarrays for Glycomic Analysis

  3. Antigen Microarrays for Immunoprofiling

    1. Front Matter

      Pages 79-79

    2. No Access

      Book Protocol

      Pages 81-104

      Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection

    3. No Access

      Book Protocol

      Pages 105-127

      SPOT Synthesis as a Tool to Study Protein–Protein Interactions

    4. No Access

      Book Protocol

      Pages 129-148

      Native Antigen Fractionation Protein Microarrays for Biomarker Discovery

    5. No Access

      Book Protocol

      Pages 149-161

      Immunoprofiling Using NAPPA Protein Microarrays

  4. Protein Function Microarrays

    1. Front Matter

      Pages 163-163

    2. No Access

      Book Protocol

      Pages 165-183

      High-Throughput Mammalian Two-Hybrid Screening for Protein–Protein Interactions Using Transfected Cell Arrays (CAPPIA)

    3. No Access

      Book Protocol

      Pages 185-200

      Protein–Protein Interactions: An Application of Tus-Ter Mediated Protein Microarray System

    4. No Access

      Book Protocol

      Pages 201-212

      Kinase Substrate Interactions

    5. No Access

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      Pages 213-223

      A Functional Protein Microarray Approach to Characterizing Posttranslational Modifications on Lysine Residues

  5. Strategies for Validation of Candidate Targets

    1. Front Matter

      Pages 225-225

    2. No Access

      Book Protocol

      Pages 227-238

      Multiplexed Detection of Antibodies Using Programmable Bead Arrays

    3. No Access

      Book Protocol

      Pages 239-254

      A Coprecipitation-Based Validation Methodology for Interactions Identified Using Protein Microarrays

  6. Generation of Proteomic Libraries

    1. Front Matter

      Pages 255-255

    2. No Access

      Book Protocol

      Pages 257-272

      Development of Expression-Ready Constructs for Generation of Proteomic Libraries

  7. Detection Methods

    1. Front Matter

      Pages 273-273

    2. No Access

      Book Protocol

      Pages 275-301

      Reverse Phase Protein Microarrays: Fluorometric and Colorimetric Detection

    3. No Access

      Book Protocol

      Pages 303-320

      Förster Resonance Energy Transfer Methods for Quantification of Protein–Protein Interactions on Microarrays

    4. No Access

      Book Protocol

      Pages 321-333

      Label-Free Detection with Surface Plasmon Resonance Imaging

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