Antiviral RNAi

Concepts, Methods, and Applications

Editors:

ISBN: 978-1-61779-036-2 (Print) 978-1-61779-037-9 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xi

  2. General Introduction

    1. Front Matter

      Pages 1-1

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      Pages 3-22

      Defense and Counterdefense in the RNAi-Based Antiviral Immune System in Insects

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      Pages 23-41

      RNAi and Cellular miRNAs in Infections by Mammalian Viruses

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      Pages 43-66

      Viral miRNAs

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      Pages 67-75

      Progress in RNAi-Based Antiviral Therapeutics

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      Pages 77-103

      Chemical Modification of Small Interfering RNA

  3. Viral Small RNAs

    1. Front Matter

      Pages 105-105

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      Pages 107-122

      Viral Small RNA Cloning and Sequencing

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      Pages 123-142

      Visitor, An Informatic Pipeline for Analysis of Viral siRNA Sequencing Datasets

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      Pages 143-152

      Computational Prediction of Viral miRNAs

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      Pages 153-171

      Detection of Viral microRNAs by Northern Blot Analysis

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      Pages 173-182

      Detection of Viral microRNA with S1 Nuclease Protection Assay

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      Pages 183-198

      Characterization of RISC-Associated Adenoviral Small RNAs

  4. RNAi-based Antiviral Defense

    1. Front Matter

      Pages 199-199

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      Pages 201-213

      Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture

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      Pages 215-229

      Dicer Assay in Drosophila S2 Cell Extract

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      Pages 231-244

      Slicer Activity in Drosophila melanogaster S2 Extract

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      Pages 245-252

      Gel Mobility Shift Assays for RNA Binding Viral RNAi Suppressors

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      Pages 253-263

      dsRNA Uptake in Adult Drosophila

  5. Antiviral RNAi Therapy

    1. Front Matter

      Pages 265-265

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      Pages 267-292

      Design of Small Interfering RNAs for Antiviral Applications

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      Pages 293-311

      RNAi-Inducing Lentiviral Vectors for Anti-HIV-1 Gene Therapy

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      Pages 313-332

      Production of Multicopy shRNA Lentiviral Vectors for Antiviral Therapy

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      Pages 333-338

      Intranasal Delivery of Antiviral siRNA

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