Polyamines

Methods and Protocols

Editors:

ISBN: 978-1-61779-033-1 (Print) 978-1-61779-034-8 (Online)

Table of contents (33 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Introduction

    1. Front Matter

      Pages 1-1

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      Book Protocol

      Pages 3-35

      Current Status of the Polyamine Research Field

  3. Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels

    1. Front Matter

      Pages 37-37

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      Book Protocol

      Pages 39-50

      Exploring Polyamine Biosynthetic Diversity Through Comparative and Functional Genomics

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      Pages 51-65

      Characterization of Genes for Polyamine Modulon

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      Pages 67-79

      Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines

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      Pages 81-111

      Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles

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      Pages 113-126

      Polyamine Block of Inwardly Rectifying Potassium Channels

  4. Transgenic Models for Study of Polyamine Function

    1. Front Matter

      Pages 127-127

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      Book Protocol

      Pages 129-141

      Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism

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      Pages 143-158

      Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism

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      Pages 159-170

      Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine

  5. Enzymes Involved in Polyamine Catabolism and Post-Translational Protein Modification

    1. Front Matter

      Pages 171-171

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      Pages 173-181

      A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease

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      Pages 183-194

      Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases

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      Pages 195-205

      Assay of Deoxyhypusine Synthase Activity

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      Pages 207-216

      Assay of Deoxyhypusine Hydroxylase Activity

  6. Regulation of Polyamine Content

    1. Front Matter

      Pages 217-217

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      Pages 219-235

      Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase

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      Pages 237-267

      Protocols for Studying Antizyme Expression and Function

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      Pages 269-278

      Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family

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      Pages 279-292

      Posttranscriptional Regulation of Ornithine Decarboxylase

  7. Polyamine Transport and Uptake

    1. Front Matter

      Pages 293-293

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      Book Protocol

      Pages 295-308

      Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae

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      Book Protocol

      Pages 309-326

      Genetic and Biochemical Analysis of Protozoal Polyamine Transporters

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      Pages 327-338

      Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake

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