The Plant Cell Wall

Methods and Protocols

Editors:

ISBN: 978-1-61779-007-2 (Print) 978-1-61779-008-9 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii

  2. No Access

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    Pages 1-20

    Plant Tissue Cultures

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    Pages 21-42

    Computerized Molecular Modeling of Carbohydrates

  4. No Access

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    Pages 43-54

    Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS

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    Pages 55-80

    High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors

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    Pages 81-92

    Carbohydrate Gel Electrophoresis

  7. No Access

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    Pages 93-102

    Capillary Electrophoresis with Detection by Laser-Induced Fluorescence

  8. No Access

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    Pages 103-113

    Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls

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    Pages 115-121

    Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays

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    Pages 123-140

    Electron Tomography and Immunogold Labelling as Tools to Analyse De Novo Assembly of Plant Cell Walls

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    Pages 141-152

    Analysing Cellulose Biosynthesis with Confocal Microscopy

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    Pages 153-167

    Visual Mapping of Cell Wall Biosynthesis

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    Pages 169-178

    Atomic Force Microscopy of Plant Cell Walls

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    Pages 179-196

    Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organisation of Primary Plant Cell Walls

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    Pages 197-208

    Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation

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    Pages 209-219

    Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis

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    Pages 221-244

    New Insights into the Control of Cell Growth

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    Pages 245-254

    Extraction and Detection of Arabinogalactan Proteins

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    Pages 255-272

    Characterization of the Plant Cell Wall Proteome Using High-Throughput Screens

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    Pages 273-290

    Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens

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    Pages 291-303

    Measuring In Vitro Extensibility of Growing Plant Cell Walls

  22. Back Matter

    Pages 305-310