Plant Immunity

Methods and Protocols

Editors:

ISBN: 978-1-61737-997-0 (Print) 978-1-61737-998-7 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xv

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    Pages 1-8

    Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression

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    Pages 9-20

    Fragment Complementation and Co-immunoprecipitation Assays for Understanding R Protein Structure and Function

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    Pages 21-30

    Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag

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    Pages 31-44

    Biochemical Purification of Native Immune Protein Complexes

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    Pages 45-58

    Chromatin Immunoprecipitation to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity

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    Pages 59-68

    Dose–Response to and Systemic Movement of Dexamethasone in the GVG-Inducible Transgene System in Arabidopsis

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    Pages 69-77

    Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis

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    Pages 79-87

    Constructing Haustorium-Specific cDNA Libraries from Rust Fungi

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    Pages 89-107

    Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes

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    Pages 109-128

    Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains

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    Pages 129-135

    A Straightforward Protocol for Electro-transformation of Phytophthora capsici Zoospores

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    Pages 137-151

    Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis: A Model Oomycete Pathogen of Arabidopsis

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    Pages 153-172

    Assaying Effector Function in Planta Using Double-Barreled Particle Bombardment

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    Pages 173-180

    Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast

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    Pages 181-194

    Purification of Effector–Target Protein Complexes via Transient Expression in Nicotiana benthamiana

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    Pages 195-209

    Imaging Fluorescently Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue

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    Pages 211-225

    Immunolocalization of Pathogen Effectors

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    Pages 227-240

    Laser Capture Microdissection of Nematode Feeding Cells

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    Pages 241-262

    Laser Microdissection of Plant–Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling

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    Pages 263-281

    Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal–Plant Interaction Sites

  22. Back Matter

    Pages 293-295

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