Regulatory T Cells

Methods and Protocols

Editors:

ISBN: 978-1-61737-978-9 (Print) 978-1-61737-979-6 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi

  2. Introduction

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-17

      Regulatory T Cells: History and Perspective

  3. In Vitro

    1. Front Matter

      Pages 19-19

    2. No Access

      Book Protocol

      Pages 21-37

      In Vitro Treg Suppression Assays

    3. No Access

      Book Protocol

      Pages 39-44

      Generation of T Cell Hybridomas from Naturally Occurring FoxP3+ Regulatory T Cells

    4. No Access

      Book Protocol

      Pages 45-54

      In Vitro and In Vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells

    5. No Access

      Book Protocol

      Pages 55-69

      Flow Cytometric Profiling of Mature and Developing Regulatory T Cells in the Thymus

    6. No Access

      Book Protocol

      Pages 71-82

      ChIP-on-Chip for FoxP3

    7. No Access

      Book Protocol

      Pages 83-101

      Live Imaging of Dendritic Cell–Treg Cell Interactions

  4. In Vivo

    1. Front Matter

      Pages 103-103

    2. No Access

      Book Protocol

      Pages 105-118

      Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In Vivo

    3. No Access

      Book Protocol

      Pages 119-156

      In Vivo Treg Suppression Assays

    4. No Access

      Book Protocol

      Pages 157-172

      In Vivo Depletion of FoxP3+ Tregs Using the DEREG Mouse Model

    5. No Access

      Book Protocol

      Pages 173-185

      Antigen-Specific Induction of Regulatory T Cells In Vivo and In Vitro

    6. No Access

      Book Protocol

      Pages 187-196

      In Vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection

  5. Human

    1. Front Matter

      Pages 197-197

    2. No Access

      Book Protocol

      Pages 199-218

      Analysis of Human FOXP3+ Treg Cells Phenotype and Function

    3. No Access

      Book Protocol

      Pages 219-231

      Depletion of Human Regulatory T Cells

    4. No Access

      Book Protocol

      Pages 233-241

      Assessment of Suppressive Capacity by Human Regulatory T Cells Using a Reproducible, Bi-Directional CFSE-Based In Vitro Assay

    5. No Access

      Book Protocol

      Pages 243-261

      Measurement of Proliferation and Disappearance of Regulatory T Cells in Human Studies Using Deuterium-Labeled Glucose

    6. No Access

      Book Protocol

      Pages 263-279

      Flow Cytometric Detection of Human Regulatory T Cells

  6. Back Matter

    Pages 281-282