Adipose-Derived Stem Cells

Methods and Protocols

Editors:

ISBN: 978-1-61737-959-8 (Print) 978-1-61737-960-4 (Online)

Table of contents (34 protocols)

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  1. Front Matter

    Pages i-xv

  2. Isolation Methods

    1. Front Matter

      Pages 1-1

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      Book Protocol

      Pages 3-16

      Isolation and Culture of Rhesus Adipose-Derived Stem Cells

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      Book Protocol

      Pages 17-27

      Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

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      Book Protocol

      Pages 29-36

      Isolation of Murine Adipose-Derived Stem Cells

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      Book Protocol

      Pages 37-46

      In Vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation

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      Book Protocol

      Pages 47-60

      In Vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics

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      Book Protocol

      Pages 61-75

      Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells

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      Book Protocol

      Pages 77-86

      Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells

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      Book Protocol

      Pages 87-105

      Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells

  3. Phenotyping Methods

    1. Front Matter

      Pages 107-107

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      Book Protocol

      Pages 109-120

      Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells

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      Pages 121-131

      Characterization of Human Adipose-Derived Stem Cells Using Flow Cytometry

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      Book Protocol

      Pages 133-150

      Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells

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      Pages 151-161

      Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants

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      Pages 163-190

      Gel-Based and Gel-Free Proteomic Technologies

  4. Differentiation Methods: In Vitro and In Vivo Applications

    1. Front Matter

      Pages 191-191

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      Book Protocol

      Pages 193-200

      Adipogenic Differentiation of Adipose-Derived Stem Cells

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      Pages 201-217

      Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells

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      Pages 219-231

      Neural Differentiation of Human Adipose Tissue-Derived Stem Cells

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      Book Protocol

      Pages 233-248

      Osteogenic Differentiation Strategies for Adipose-Derived Mesenchymal Stem Cells

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      Book Protocol

      Pages 249-260

      Generation of Adipose Stromal Cell-Derived Hepatic Cells

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      Book Protocol

      Pages 261-268

      Adipose Stem Cell Differentiation into Smooth Muscle Cells

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      Book Protocol

      Pages 269-287

      Endothelial and Cardiac Regeneration from Adipose Tissues

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