Flow Cytometry Protocols

Editors:

ISBN: 978-1-61737-949-9 (Print) 978-1-61737-950-5 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-29

    Flow Cytometry: An Introduction

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    Pages 31-51

    Breaking the Dimensionality Barrier

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    Pages 53-65

    Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

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    Pages 67-84

    Quantum Dots for Quantitative Flow Cytometry

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    Pages 85-95

    Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry

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    Pages 97-118

    Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization

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    Pages 119-164

    Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry

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    Pages 165-178

    Multiparameter Intracellular Cytokine Staining

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    Pages 179-202

    Phospho Flow Cytometry Methods for the Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples

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    Pages 203-227

    Multiparametric Analysis of Apoptosis by Flow Cytometry

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    Pages 229-249

    Multiparameter Cell Cycle Analysis

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    Pages 251-273

    Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue

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    Pages 275-293

    Flow Cytometry-Based Identification of Immature Myeloerythroid Development

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    Pages 295-316

    Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia

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    Pages 317-335

    Flow Cytometry Assays in Primary Immunodeficiency Diseases

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    Pages 337-354

    Flow Cytometric Analysis of Microparticles

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    Pages 355-370

    Noncytotoxic DsRed Derivatives for Whole-Cell Labeling

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    Pages 371-392

    Flow Cytometric FRET Analysis of Protein Interaction

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    Pages 393-405

    Fluorescent Protein-Assisted Purification for Gene Expression Profiling

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    Pages 407-429

    Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type

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