Quorum Sensing

Methods and Protocols

Editors:

ISBN: 978-1-60761-970-3 (Print) 978-1-60761-971-0 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xvi

  2. Detection, Isolation, and Characterization of Quorum Sensing Compounds

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-19

      Bioassays of Quorum Sensing Compounds Using Agrobacterium tumefaciens and Chromobacterium violaceum

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      Pages 21-30

      Detection of 2-Alkyl-4-Quinolones Using Biosensors

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      Pages 31-46

      FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds

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      Pages 47-59

      Isolation of agr Quorum Sensing Autoinducers

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      Pages 61-69

      Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of N-Acyl-l-Homoserine Lactones and 4-Hydroxy-2-Alkylquinolines

    7. No Access

      Book Protocol

      Pages 71-82

      Detection of Autoinducer (AI-2)-Like Activity in Food Samples

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      Pages 83-100

      Detection of Bacterial Signaling Molecules in Liquid or Gaseous Environments

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      Pages 101-111

      Determination of Acyl Homoserine Lactone and Tetramic Acid Concentrations in Biological Samples

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      Pages 113-130

      Luminescent Reporters and Their Applications for the Characterization of Signals and Signal-Mimics that Alter LasR-Mediated Quorum Sensing

  3. Determining the Function of Autoinducers In vivo

    1. Front Matter

      Pages 131-131

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      Book Protocol

      Pages 133-145

      Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules

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      Pages 147-157

      Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

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      Pages 159-171

      Defining the Structure and Function of Acyl-Homoserine Lactone Autoinducers

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      Pages 173-187

      Global Expression Analysis of Quorum-Sensing Controlled Genes

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      Pages 189-206

      Small RNA Target Genes and Regulatory Connections in the Vibrio cholerae Quorum Sensing System

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      Pages 207-217

      Quantifying Pseudomonas aeruginosa Quinolones and Examining Their Interactions with Lipids

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      Pages 219-233

      Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans

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      Pages 235-249

      Design of Synthetic Mammalian Quorum-Sensing Systems

  4. Quorum Sensing Disruption Strategies

    1. Front Matter

      Pages 251-251

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      Book Protocol

      Pages 253-263

      Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro

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      Pages 265-274

      Custom Synthesis of Autoinducers and Their Analogues

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      Pages 275-290

      Heterologous Overexpression, Purification, and In Vitro Characterization of AHL Lactonases

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