Book Volume 690 2011

Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

Methods and Protocols

Editors:

ISBN: 978-1-60761-961-1 (Print) 978-1-60761-962-8 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xii

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    Protocol

    Pages 1-30

    Embryonic Stem Cells for Osteo-Degenerative Diseases

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    Protocol

    Pages 31-56

    Methods for Culturing Mouse and Human Embryonic Stem Cells

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    Protocol

    Pages 57-66

    Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells

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    Protocol

    Pages 67-80

    Functional Assays for Human Embryonic Stem Cell Pluripotency

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    Protocol

    Pages 81-94

    Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells

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    Protocol

    Pages 95-106

    Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters

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    Protocol

    Pages 107-120

    Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives

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    Protocol

    Pages 121-134

    Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers

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    Protocol

    Pages 135-149

    Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology

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    Protocol

    Pages 151-162

    Methods for Embryoid Body Formation: The Microwell Approach

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    Protocol

    Pages 163-174

    Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview

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    Protocol

    Pages 175-182

    Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells

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    Protocol

    Pages 183-193

    Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the “Raclure” Method

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    Protocol

    Pages 195-215

    Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes

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    Protocol

    Pages 217-237

    Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds

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    Protocol

    Pages 239-253

    Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells

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    Protocol

    Pages 255-272

    Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield

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    Protocol

    Pages 273-284

    Identification of Osteoclasts in Culture

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    Protocol

    Pages 285-300

    Analysis of Glycosaminoglycans in Stem Cell Glycomics

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    Protocol

    Pages 301-314

    Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent

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