PCR Protocols

Editors:

ISBN: 978-1-60761-943-7 (Print) 978-1-60761-944-4 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xi

  2. PCR Husbandry

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-14

      Setup of a PCR Laboratory

  3. Cloning and Sequencing

    1. Front Matter

      Pages 15-15

    2. No Access

      Book Protocol

      Pages 17-23

      Long-Range PCR with a DNA Polymerase Fusion

    3. No Access

      Book Protocol

      Pages 25-42

      Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures

    4. No Access

      Book Protocol

      Pages 43-55

      Lariat-Dependent Nested PCR for Flanking Sequence Determination

    5. No Access

      Book Protocol

      Pages 57-73

      CODEHOP PCR and CODEHOP PCR Primer Design

    6. No Access

      Book Protocol

      Pages 75-88

      Sequencing of Difficult DNA Regions by SAM Sequencing

  4. Expression, Epigenetic and Copy Number Analysis

    1. Front Matter

      Pages 89-89

    2. No Access

      Book Protocol

      Pages 91-111

      A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis

    3. No Access

      Book Protocol

      Pages 113-134

      Quantitation of MicroRNAs by Real-Time RT-qPCR

    4. No Access

      Book Protocol

      Pages 135-146

      High-Throughput SuperSAGE

    5. No Access

      Book Protocol

      Pages 147-163

      Deep Cap Analysis of Gene Expression

    6. No Access

      Book Protocol

      Pages 165-175

      Linking Emulsion PCR Haplotype Analysis

    7. No Access

      Book Protocol

      Pages 177-192

      PAP-LMPCR: An Improved, Sequence-Selective Method for the In Vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure

    8. No Access

      Book Protocol

      Pages 193-205

      The Many Faces of MLPA

    9. No Access

      Book Protocol

      Pages 207-217

      Assessing Gene-Specific Methylation Using HRM-Based Analysis

  5. Probes and Assays

    1. Front Matter

      Pages 219-219

    2. No Access

      Book Protocol

      Pages 221-229

      Alu PCR

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      Book Protocol

      Pages 231-243

      Asynchronous PCR

    4. No Access

      Book Protocol

      Pages 245-256

      Novel Applications of PCR Through the Use of DNA Substrates

    5. No Access

      Book Protocol

      Pages 257-264

      Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support

    6. No Access

      Book Protocol

      Pages 265-274

      Application of Blocking Oligonucleotides to Improve Signal-to-Noise Ratio in a PCR

  6. Engineering and Screening

    1. Front Matter

      Pages 275-275

    2. No Access

      Book Protocol

      Pages 277-282

      Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis

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