Drug Safety Evaluation

Methods and Protocols

Editors:

ISBN: 978-1-60327-186-8 (Print) 978-1-60761-849-2 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-xiii

  2. General Toxicology

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-16

      Developing Combination Drugs in Preclinical Studies

    3. No Access

      Book Protocol

      Pages 17-35

      Preclinical Evaluation of Juvenile Toxicity

  3. Pathology

    1. Front Matter

      Pages 37-37

    2. No Access

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      Pages 39-67

      Necropsy and Sampling Procedures in Rodents

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      Pages 69-82

      Histopathology Procedures: From Tissue Sampling to Histopathological Evaluation

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      Pages 83-96

      Principles and Methods of Immunohistochemistry

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      Pages 97-112

      Tissue Microarrays and Digital Image Analysis

  4. Genetic Toxicology

    1. Front Matter

      Pages 113-113

    2. No Access

      Book Protocol

      Pages 115-136

      Micronucleus Assay and Labeling of Centromeres with FISH Technique

    3. No Access

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      Pages 137-147

      The Use of Bacterial Repair Endonucleases in the Comet Assay

  5. Safety Pharmacology

    1. Front Matter

      Pages 149-149

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      Pages 151-163

      Manual Whole-Cell Patch-Clamping of the HERG Cardiac K+ Channel

  6. Generation and Analysis of Transcriptomics Data

    1. Front Matter

      Pages 165-165

    2. No Access

      Book Protocol

      Pages 167-185

      Generation and Analysis of Transcriptomics Data

    3. No Access

      Book Protocol

      Pages 187-203

      Protocols of Two-Dimensional Difference Gel Electrophoresis to Investigate Mechanisms of Toxicity

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      Book Protocol

      Pages 205-225

      Protocols and Applications of Cellular Metabolomics in Safety Studies Using Precision-Cut Tissue Slices and Carbon 13 NMR

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      Pages 227-241

      Statistical Analysis of Quantitative RT-PCR Results

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      Pages 243-253

      Evaluation of Mitochondrial Respiration in Cultured Rat Hepatocytes

  7. Screening Assays for Developmental Toxicity

    1. Front Matter

      Pages 255-255

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      Book Protocol

      Pages 257-269

      FETAX Assay for Evaluation of Developmental Toxicity

    3. No Access

      Book Protocol

      Pages 271-279

      Evaluation of Embryotoxicity Using the Zebrafish Model

  8. Chemical Protein Adducts

    1. Front Matter

      Pages 281-281

    2. No Access

      Book Protocol

      Pages 283-301

      Protocols of In Vitro Protein Covalent Binding Studies in Liver

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      Pages 303-316

      Utilization of MALDI-TOF to Determine Chemical-Protein Adduct Formation In Vitro

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      Pages 317-326

      Utilization of LC-MS/MS Analyses to Identify Site-Specific Chemical Protein Adducts In Vitro

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      Pages 327-338

      One-Dimensional Western Blotting Coupled to LC-MS/MS Analysis to Identify Chemical-Adducted Proteins in Rat Urine

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