Plant Developmental Biology

Methods and Protocols

Editors:

ISBN: 978-1-60761-764-8 (Print) 978-1-60761-765-5 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-10

    Growth Protocols for Model Plants in Developmental Biology

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    Pages 11-26

    Grafting as a Research Tool

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    Pages 27-45

    Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function

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    Pages 47-64

    The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level

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    Pages 65-75

    Inducible Gene Expression Systems for Plants

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    Pages 77-88

    Trichome Development in Arabidopsis

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    Pages 89-103

    Phenotyping the Development of Leaf Area in Arabidopsis thaliana

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    Pages 105-129

    Analyzing Shoot Apical Meristem Development

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    Pages 131-142

    Analyzing Floral Meristem Development

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    Pages 143-153

    Female Gametophytic Mutants: Diagnosis and Characterization

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    Pages 155-176

    Pollen Tube Development

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    Pages 177-187

    Analysis of Root Meristem Size Development

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    Pages 189-202

    Phenotypic Characterization of Photomorphogenic Responses During Plant Development

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    Pages 203-227

    Kinematic Analysis of Cell Division and Expansion

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    Pages 229-237

    Flowering Time Control

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    Pages 239-251

    mRNA Detection by Whole Mount In Situ Hybridization (WISH) or Sectioned Tissue In Situ Hybridization (SISH) in Arabidopsis

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    Pages 253-263

    Immunolocalization of Proteins in Plants

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    Pages 265-274

    Detection of Small Non-coding RNAs

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    Pages 275-291

    Quantitative Real Time PCR in Plant Developmental Biology

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    Pages 293-312

    Luciferase and Green Fluorescent Protein Reporter Genes as Tools to Determine Protein Abundance and Intracellular Dynamics

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