Hepatocytes

Methods and Protocols

Editors:

ISBN: 978-1-60761-687-0 (Print) 978-1-60761-688-7 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xix

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    Pages 1-40

    General Review on In Vitro Hepatocyte Models and Their Applications

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    Pages 41-55

    Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation

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    Pages 57-82

    Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue

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    Pages 83-105

    Optimisation of the Cryopreservation of Primary Hepatocytes

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    Pages 107-113

    Cryopreservation of Human Hepatocytes for Clinical Use

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    Pages 115-138

    Hepatocyte Differentiation

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    Pages 139-155

    Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix-Mediated Manipulation of Signaling Activities

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    Pages 157-166

    Markers and Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies

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    Pages 167-179

    Hepatic Stem Cells

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    Pages 181-236

    Hepatic Stem Cells and Liver Development

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    Pages 237-246

    Generation of Hepatocytes from Human Embryonic Stem Cells

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    Pages 247-260

    Isolation and Culture of Adult Human Liver Progenitor Cells: In Vitro Differentiation to Hepatocyte-Like Cells

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    Pages 261-272

    The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies

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    Pages 273-280

    Transdifferentiation of Pancreatic Cells to Hepatocytes

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    Pages 281-294

    Evaluation of Drug Metabolism, Drug–Drug Interactions, and In Vitro Hepatotoxicity with Cryopreserved Human Hepatocytes

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    Pages 295-308

    The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction

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    Pages 309-326

    The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases

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    Pages 327-353

    The Use of Hepatocytes to Investigate Drug Uptake Transporters

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    Pages 355-374

    Metabonomic Studies on Human Hepatocyte in Primary Culture

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    Pages 375-387

    The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds

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