RNA Interference

From Biology to Clinical Applications

Editors:

ISBN: 978-1-60761-587-3 (Print) 978-1-60761-588-0 (Online)

Table of contents (27 protocols)

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  1. Front Matter

    Pages i-xvi

  2. Physiology of RNA Interference

    1. Front Matter

      Pages 1-1

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      Pages 3-19

      Endogenous Antiviral Mechanisms of RNA Interference: A Comparative Biology Perspective

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      Pages 21-33

      Monitoring Innate Immune Recruitment by siRNAs in Mammalian Cells

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      Pages 35-65

      Current Knowledge of MicroRNAs and Noncoding RNAs in Virus-Infected Cells

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      Pages 67-79

      Allele-Specific Silencing by RNA Interference

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      Pages 81-92

      Computational siRNA Design Considering Alternative Splicing

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      Pages 93-107

      Bioinformatic Approaches to siRNA Selection and Optimization

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      Pages 109-121

      Optimized Gene Silencing by Co-expression of Multiple shRNAs in a Single Vector

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      Pages 123-136

      Strategies in Designing Multigene Expression Units to Downregulate HIV-1

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      Pages 137-154

      Designing Optimal siRNA Based on Target Site Accessibility

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      Pages 155-170

      Chemical Synthesis of 2′-O-Alkylated siRNAs

  3. RNA Interference in the Laboratory and siRNA Delivery

    1. Front Matter

      Pages 172-172

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      Pages 173-188

      siRNA Specific Delivery System for Targeting Dendritic Cells

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      Pages 189-195

      Hydrodynamic Delivery Protocols

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      Pages 197-209

      New Methods for Reverse Transfection with siRNA from a Solid Surface

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      Pages 211-229

      Nonviral siRNA Delivery for Gene Silencing in Neurodegenerative Diseases

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      Pages 231-242

      Using siRNA to Uncover Novel Oncogenic Signaling Pathways

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      Pages 243-269

      Biodegradable Polymer-Mediated sh/siRNA Delivery for Cancer Studies

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      Pages 271-281

      Cellular siRNA Delivery Using TatU1A and Photo-Induced RNA Interference

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      Pages 283-297

      Polyethylenimine (PEI)/siRNA-Mediated Gene Knockdown In Vitro and In Vivo

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      Pages 299-309

      Transfection of siRNAs in Multiple Myeloma Cell Lines

  4. Clinical Implementation

    1. Front Matter

      Pages 312-312

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      Pages 313-324

      A New Approach for Therapeutic Use by RNA Interference in the Brain

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