T-Cell Trafficking

Methods and Protocols

Editors:

ISBN: 978-1-60761-460-9 (Print) 978-1-60761-461-6 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii

  2. Introductory Review

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-13

      How T Cells Find Their Way Around

  3. Migration of T Cells In Vitro

    1. Front Matter

      Pages 15-15

    2. No Access

      Book Protocol

      Pages 17-30

      Live Imaging of Leukocyte–Endothelium Interactions

    3. No Access

      Book Protocol

      Pages 31-47

      Leucocyte Adhesion Under Haemodynamic Flow Conditions

    4. No Access

      Book Protocol

      Pages 49-68

      Influence of Stromal Cells on Lymphocyte Adhesion and Migration on Endothelial Cells

    5. No Access

      Book Protocol

      Pages 69-82

      Discriminating Between the Paracellular and Transcellular Routes of Diapedesis

    6. No Access

      Book Protocol

      Pages 83-95

      Monitoring RhoGTPase Activity in Lymphocytes

    7. No Access

      Book Protocol

      Pages 97-113

      Visualisation of Signalling in Immune Cells

    8. No Access

      Book Protocol

      Pages 115-124

      Methods for Quantitation of Leukocyte Chemotaxis and Fugetaxis

    9. No Access

      Book Protocol

      Pages 125-147

      Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes

    10. No Access

      Book Protocol

      Pages 149-158

      Transfection of Indoleamine 2,3 Dioxygenase in Primary Endothelial Cells

  4. Migration of T Cells In Vivo

    1. Front Matter

      Pages 159-159

    2. No Access

      Book Protocol

      Pages 161-179

      Visualisation of Lymphoid Organ Development

    3. No Access

      Book Protocol

      Pages 181-192

      Single-Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy

    4. No Access

      Book Protocol

      Pages 193-206

      Imaging Interactions Between the Immune and Cardiovascular Systems In Vivo by Multiphoton Microscopy

    5. No Access

      Book Protocol

      Pages 207-228

      Applying an Adaptive Watershed to the Tissue Cell Quantification During T-Cell Migration and Embryonic Development

  5. Monitoring T-Cell Migration in Human Diseases

    1. Front Matter

      Pages 229-229

    2. No Access

      Book Protocol

      Pages 231-252

      Identifying Homing Interactions in T-Cell Traffic in Human Disease

    3. No Access

      Book Protocol

      Pages 253-266

      Tracking Antigen-Experienced Effector T Cells In Vitro and In Vivo

    4. No Access

      Book Protocol

      Pages 267-280

      Preclinical Testing of Strategies for Therapeutic Targeting of Human T-Cell Trafficking In Vivo

  6. Back Matter

    Pages 281-290