Dendritic Cell Protocols

Editors:

ISBN: 978-1-60761-420-3 (Print) 978-1-60761-421-0 (Online)

Table of contents (28 protocols)

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  1. Front Matter

    Pages i-xvii

  2. Part I: Reviews

    1. Front Matter

      Pages 1-1

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      Pages 3-20

      Review of Human DC Subtypes

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      Pages 21-42

      Review of Murine Dendritic Cells: Types, Location, and Development

  3. Part II: Human DC Protocols

    1. Front Matter

      Pages 43-43

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      Pages 45-54

      Isolation of Human Blood DC Subtypes

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      Pages 55-65

      Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin

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      Pages 67-85

      Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells

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      Pages 87-115

      In Vivo Modulation of Gene Expression by Lentiviral Transduction in “Human Immune System” Rag2−/−γc −/− Mice

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      Pages 117-133

      Generation of Stable Th1/CTL-, Th2-, and Th17-Inducing Human Dendritic Cells

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      Pages 135-148

      Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation

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      Pages 149-163

      Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines

  4. Part III: Murine Dendritic Cell Methods

    1. Front Matter

      Pages 165-165

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      Pages 167-176

      CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand

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      Pages 177-186

      Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro

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      Pages 187-193

      The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF

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      Pages 195-203

      Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow

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      Pages 205-229

      The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry

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      Pages 231-233

      Isolation of Cutaneous Dendritic Cells

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      Pages 235-248

      Isolation of Skin Dendritic Cells from Mouse and Man

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      Pages 249-263

      Identification and Isolation of Rodent Respiratory Tract Dendritic Cells

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      Pages 265-280

      Isolation of Mouse Thymic Dendritic Cell Precursors

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      Pages 281-297

      Isolation of Rat Intestinal Lymph DC

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      Pages 299-318

      Mouse Models of Viral Infection: Influenza Infection in the Lung

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