Advanced Protocols in Oxidative Stress II

Editors:

ISBN: 978-1-60761-410-4 (Print) 978-1-60761-411-1 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Reactive Oxygen and Nitrogen Techniques

    1. Front Matter

      Pages 1-1

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      Pages 3-17

      Current Status of Measuring Oxidative Stress

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      Pages 19-41

      pO2 and ROS/RNS Measurements in the Microcirculation in Hypoxia

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      Pages 43-55

      Nitrate Reductase Activity of Mitochondrial Aldehyde Dehydrogenase (ALDH-2) as a Redox Sensor for Cardiovascular Oxidative Stress

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      Pages 57-72

      Identification of ROS Using Oxidized DCFDA and Flow-Cytometry

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      Pages 73-84

      Measurement of 8-Isoprostane in Exhaled Breath Condensate

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      Pages 85-105

      Electron Paramagnetic Resonance Oximetry and Redoximetry

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      Pages 107-121

      Measurement of Mitochondrial Membrane Potential and Proton Leak

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      Pages 123-128

      Determination of Erythrocyte Fragility as a Marker of Pesticide-Induced Membrane Oxidative Damage

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      Pages 129-140

      Using N,N,N’,N’-tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro

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      Pages 141-153

      Structural and Functional Changes in the Insulin Molecule Produced by Oxidative Stress

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      Pages 155-162

      Multiphoton Redox Ratio Imaging for Metabolic Monitoring In Vivo

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      Pages 163-171

      Using Fluorescence-Activated Flow Cytometry to Determine Reactive Oxygen Species Formation and Membrane Lipid Peroxidation in Viable Boar Spermatozoa

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      Pages 173-193

      Lipofuscin: Detection and Quantification by Microscopic Techniques

  3. Antioxidant Technology and Application

    1. Front Matter

      Pages 196-196

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      Pages 197-213

      OXY-SCORE: A Global Index to Improve Evaluation of Oxidative Stress by Combining Pro- and Antioxidant Markers

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      Pages 215-239

      Cupric Ion Reducing Antioxidant Capacity Assay for Antioxidants in Human Serum and for Hydroxyl Radical Scavengers

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      Pages 241-250

      Analysis of Antioxidant Activities in Vegetable Oils and Fat Soluble Vitamins and Biofactors by the PAO-SO Method

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      Pages 251-262

      Measuring Antioxidant Capacity Using the ORAC and TOSC Assays

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      Pages 263-273

      Assessing the Neuroprotective Effect of Antioxidant Food Factors by Application of Lipid-Derived Dopamine Modification Adducts

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      Pages 275-285

      LIBS-Based Detection of Antioxidant Elements: A New Strategy

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      Pages 287-296

      A Method for Evaluation of Antioxidant Activity Based on Inhibition of Free Radical-Induced Erythrocyte Hemolysis

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