Cytoskeleton Methods and Protocols

Editors:

ISBN: 978-1-60761-375-6 (Print) 978-1-60761-376-3 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xi

  2. Live-Cell Imaging of the Cytoskeleton

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-21

      High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis

    3. No Access

      Book Protocol

      Pages 23-39

      Imaging the Cytoskeleton in Live Xenopus laevis Embryos

    4. No Access

      Book Protocol

      Pages 41-68

      Live-Cell Imaging of the Cytoskeleton and Mitochondrial–Cytoskeletal Interactions in Budding Yeast

  3. Dynamics of Actin Filaments and Microtubules

    1. Front Matter

      Pages 70-70

    2. No Access

      Book Protocol

      Pages 71-87

      Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization

    3. No Access

      Book Protocol

      Pages 89-113

      Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts

  4. Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components

    1. Front Matter

      Pages 116-116

    2. No Access

      Book Protocol

      Pages 117-142

      Quantitative Fluorescence Microscopy Techniques

    3. No Access

      Book Protocol

      Pages 143-156

      A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule

    4. No Access

      Book Protocol

      Pages 157-169

      Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots

    5. No Access

      Book Protocol

      Pages 171-184

      Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells

  5. Electron Microscopy Protocols for Imaging the Cytoskeleton

    1. Front Matter

      Pages 186-186

    2. No Access

      Book Protocol

      Pages 187-206

      Imaging Cytoskeleton Components by Electron Microscopy

    3. No Access

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      Pages 207-225

      Purification and Localization of Intraflagellar Transport Particles and Polypeptides

    4. No Access

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      Pages 227-241

      High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies

    5. No Access

      Book Protocol

      Pages 243-262

      Strategies for Imaging Microtubules in Plant Cells

  6. Analysis of Cell and Organelle Motility

    1. Front Matter

      Pages 264-264

    2. No Access

      Book Protocol

      Pages 265-273

      A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity

    3. No Access

      Book Protocol

      Pages 275-282

      Melanosome Motility in Fish Retinal Pigment Epithelial Cells

    4. No Access

      Book Protocol

      Pages 283-299

      Analysis of Properties of Cilia Using Tetrahymena thermophila

    5. No Access

      Book Protocol

      Pages 301-313

      Application of Cell Traction Force Microscopy for Cell Biology Research

    6. No Access

      Book Protocol

      Pages 315-335

      2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics

    7. No Access

      Book Protocol

      Pages 337-353

      Detergent-Extracted Models for the Study of Cilia or Flagella

  7. Genetic and Proteomics Approaches for Cytoskeleton Protein Function

    1. Front Matter

      Pages 356-356

    2. No Access

      Book Protocol

      Pages 357-365

      The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions

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