Lipidomics

Volume 2: Methods and Protocols

Editors:

ISBN: 978-1-60761-324-4 (Print) 978-1-60761-325-1 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii

  2. Oxidized Lipids

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-16

      F2-Isoprostanes: Sensitive Biomarkers of Oxidative Stress In Vitro and In Vivo: A Gas Chromatography-Mass Spectrometric Approach

    3. No Access

      Book Protocol

      Pages 17-28

      Volatile Oxylipins and Related Compounds Formed Under Stress in Plants

    4. No Access

      Book Protocol

      Pages 29-37

      Quantification of Lysophosphatidylcholine Species by High-Throughput Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)

    5. No Access

      Book Protocol

      Pages 39-91

      Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography, HPLC, and On-line MS

    6. No Access

      Book Protocol

      Pages 93-107

      Lipid Raft-Redox Signaling Platforms in Plasma Membrane

    7. No Access

      Book Protocol

      Pages 109-128

      Mass Spectrometry Analysis of Polyisoprenoids Alcohols and Carotenoids via ESI(Li+)-MS/MS

    8. No Access

      Book Protocol

      Pages 129-141

      Detection of a Lipid-Lysine Adduct Family with an Amide Bond as the Linkage: Novel Markers for Lipid-Derived Protein Modifications

    9. No Access

      Book Protocol

      Pages 143-152

      Assessing the Neuroprotective Effect of Antioxidative Food Factors by Application of Lipid-Derived Dopamine Modification Adducts

    10. No Access

      Book Protocol

      Pages 153-183

      Mass-Spectrometric Characterization of Phospholipids and Their Hydroperoxide Derivatives In Vivo: Effects of Total Body Irradiation

  3. Trafficking and Profiling

    1. Front Matter

      Pages 186-186

    2. No Access

      Book Protocol

      Pages 187-201

      Comprehensive mRNA Profiling of Lipid-Related Genes in Microglia and Macrophages Using Taqman Arrays

    3. No Access

      Book Protocol

      Pages 203-220

      Imaging Lipid Membrane Domains with Lipid-Specific Probes

    4. No Access

      Book Protocol

      Pages 221-232

      Monitoring Sterol Uptake, Acetylation, and Export in Yeast

    5. No Access

      Book Protocol

      Pages 233-249

      Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation

    6. No Access

      Book Protocol

      Pages 251-266

      Activity-Based Profiling of Lipases in Living Cells

    7. No Access

      Book Protocol

      Pages 267-281

      Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-Related Maculopathy

  4. Software and Bioinformatics

    1. Front Matter

      Pages 284-284

    2. No Access

      Book Protocol

      Pages 285-294

      Instrument-Independent Software Tools for the Analysis of MS–MS and LC–MS Lipidomics Data

    3. No Access

      Book Protocol

      Pages 295-316

      Computer-Assisted Interpretation of Triacylglycerols Mass Spectra

    4. No Access

      Book Protocol

      Pages 317-338

      Visualization of Complex Processes in Lipid Systems Using Computer Simulations and Molecular Graphics

    5. No Access

      Book Protocol

      Pages 339-368

      Bioinformatics Strategies for the Analysis of Lipids

  5. Biostatistics

    1. Front Matter

      Pages 370-370

    2. No Access

      Book Protocol

      Pages 371-381

      The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Human Health Risks

  6. Back Matter

    Pages 383-390