Protocols for Neural Cell Culture

Fourth Edition

Editors:

ISBN: 978-1-60761-291-9 (Print) 978-1-60761-292-6 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-28

    Neurosphere and Neural Colony-Forming Cell Assays

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    Pages 29-49

    Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells

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    Pages 51-73

    Culture and Differentiation of Human Neural Stem Cells

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    Pages 75-86

    Neural Differentiation of Human Embryonic Stem Cells

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    Pages 87-104

    Isolation and Culture of Primary Human CNS Neural Cells

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    Pages 105-123

    Bioengineering Protocols for Neural Precursor Cell Expansion

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    Pages 125-136

    Intracellular Calcium Assays in Dissociated Primary Cortical Neurons

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    Pages 137-159

    Dissociated Hippocampal Cultures

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    Pages 161-173

    Primary Sensory and Motor Neuron Cultures

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    Pages 175-191

    Retinal Cell and Tissue Culture

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    Pages 193-215

    Preparation of Normal and Reactive Astrocyte Cultures

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    Pages 217-230

    Oligodendrocyte Progenitor Cell Culture

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    Pages 231-239

    Isolation of Microglia Subpopulations

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    Pages 241-251

    Microglia from Progenitor Cells in Mouse Neopallium

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    Pages 253-268

    Primary Schwann Cell Cultures

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    Pages 269-283

    Primary Dissociated Astrocyte and Neuron Co-culture

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    Pages 285-298

    Cerebellar Slice Cultures

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    Pages 299-311

    Hippocampal Slice Cultures

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    Pages 313-327

    Molecular Substrates for Growing Neurons in Culture

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    Pages 329-341

    Guidance and Outgrowth Assays for Embryonic Thalamic Axons

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