Molecular Epidemiology of Microorganisms

Methods and Protocols

Editors:

ISBN: 978-1-60327-998-7 (Print) 978-1-60327-999-4 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-12

    Microbial Molecular Epidemiology: An Overview

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    Pages 13-25

    Multilocus Enzyme Electrophoresis for Parasites and Other Pathogens

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    Pages 27-35

    Plasmid Replicon Typing

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    Pages 37-47

    The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms

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    Pages 49-58

    Use of Repetitive Element Palindromic PCR (rep-PCR) for the Epidemiologic Discrimination of Foodborne Pathogens

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    Pages 59-70

    Pulsed-Field Gel Electrophoresis for Molecular Epidemiology of Food Pathogens

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    Pages 71-87

    Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance

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    Pages 89-104

    Amplified Fragment Length Polymorphism Analysis

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    Pages 105-116

    Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens

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    Pages 117-128

    Spoligotyping for Molecular Epidemiology of the Mycobacterium tuberculosis Complex

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    Pages 129-140

    Multilocus Sequence Typing

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    Pages 141-158

    Multiple Locus Variable Number of Tandem Repeats Analysis

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    Pages 159-171

    Comparison of Molecular Typing Methods Applied to Clostridium difficile

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    Pages 173-188

    Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis

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    Pages 189-202

    spa Typing for Epidemiological Surveillance of Staphylococcus aureus

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    Pages 203-215

    Sequencing of Viral Genes

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    Pages 217-230

    Full Sequencing of Viral Genomes: Practical Strategies Used for the Amplification and Characterization of Foot-and-Mouth Disease Virus

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    Pages 231-247

    Bacterial Genome Sequencing

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    Pages 249-285

    DNA Microarray for Molecular Epidemiology of Salmonella

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    Pages 287-304

    Methods for Data Analysis

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