Tumor Biomarker Discovery

Methods and Protocols

Editors:

ISBN: 978-1-60327-810-2 (Print) 978-1-60327-811-9 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-10

    Identification of Tumor-Associated Antigens as Diagnostic and Predictive Biomarkers in Cancer

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    Pages 11-19

    Autoantibodies Against Cancer Antigens

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    Pages 21-38

    Discovery of Antibody Biomarkers Using Protein Microarrays of Tumor Antigens Cloned in High Throughput

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    Pages 39-58

    Analysis of Glycans on Serum Proteins Using Antibody Microarrays

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    Pages 59-74

    Glycoproteomic Analysis by Two-Dimensional Electrophoresis

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    Pages 75-87

    All-Liquid Separations, Protein Microarrays, and Mass Spectrometry to Interrogate Serum Proteomes: An Application to Serum Glycoproteomics

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    Pages 89-105

    Reverse-Phase Protein Microarrays for Theranostics and Patient Tailored Therapy

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    Pages 107-128

    Serum Proteomics Using Mass Spectrometry

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    Pages 129-141

    Hormones as Biomarkers: Practical Guide to Utilizing Luminex Technologies for Biomarker Research

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    Pages 143-150

    High-Throughput Analysis of Serum Antigens Using Sandwich ELISAs on Microarrays

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    Pages 151-161

    Tissue Microarrays as a Tool in the Discovery and Validation of Tumor Markers

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    Pages 163-175

    Quantitative, Fluorescence-Based In-Situ Assessment of Protein Expression

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    Pages 177-193

    Tumor Marker Discovery by Expression Profiling RNA from Formalin Fixed Paraffin Embedded Tissues

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    Pages 195-204

    High-Throughput Mutation Screening Using a Single Amplification Condition

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    Pages 205-220

    DNA Sequencing of Cancer-Related Genes for Biomarker Discovery

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    Pages 221-229

    Analysis of Loss of Heterozygosity in Circulating DNA

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    Pages 231-245

    Pharmacogenomics

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    Pages 247-257

    Study Designs in Genetic Epidemiology

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    Pages 259-272

    Developing Classifiers for the Detection of Cancer Using Multi-Analytes

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    Pages 273-295

    Metabolomics of Cancer

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