Advanced Protocols in Oxidative Stress I

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ISBN: 978-1-60327-218-6 (Print) 978-1-60327-517-0 (Online)

Table of contents (31 protocols)

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  1. Front Matter

    Pages i-xiii

  2. Reactive Oxygen and Nitrogen Techniques

    1. Front Matter

      Pages 1-1

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      Pages 3-13

      Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cells, and Tissues

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      Pages 15-29

      A Rapid and Selective Mass Spectrometric Method for the Identification of Nitrated Proteins

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      Pages 31-39

      An Easy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting

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      Pages 41-48

      Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization in Studies of Oxidative and Nitrosative Stress

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      Pages 49-63

      Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL

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      Pages 65-73

      High Performance Liquid Chromatography/Electron Spin Resonance/Mass Spectrometry Analyses of Lipid-Derived Radicals

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      Pages 75-88

      EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/OOH and Immunospin-Trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer System

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      Pages 89-97

      Determination of High Mitochondrial Membrane Potential in Spermatozoa Loaded with the Mitochondrial Probe 5,5′,6,6′-Tetrachloro-1,1′,3,3′-Tetraethylbenzimidazolyl-Carbocyanine Iodide (JC-1) by Using Fluorescence-Activated Flow Cytometry

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      Pages 99-112

      2,2,6,6-Tetramethylpiperidin-1-Oxyl Probes for Evaluating Oxidative Stress on the Cell Membrane and Mitochondria

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      Pages 113-121

      Zymographical Techniques for Detectionof Matrix Metalloproteinases

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      Pages 123-136

      The Use of In Vivo Microdialysis Techniques to Detect Extracellular ROS in Resting and Contracting Skeletal Muscle

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      Pages 137-147

      Cell-Free Antibody Capture Method for Analysis of Detergent-Resistant Membrane Rafts

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      Pages 149-160

      Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids

  3. Antioxidant Technology and Application

    1. Front Matter

      Pages 161-161

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      Pages 163-193

      Cupric Ion Reducing Antioxidant Capacity Assay for Food Antioxidants: Vitamins, Polyphenolics, and Flavonoids in Food Extracts

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      Pages 195-206

      Redox Property of Ribonucleotide ReductaseSmall Subunit M2 and p53R2

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      Pages 207-218

      Antioxidant QSAR Modeling as Exemplified on Polyphenols

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      Pages 219-228

      Immunohistochemical Staining of Cyclooxygenases with Monoclonal Antibodies

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      Pages 229-243

      Examining the Endogenous Antioxidant Response Through Immunofluorescent Analysis of Nrf2 in Tissue

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      Pages 245-258

      Determination of Oxidized and Reduced CoQ10 and CoQ9 in Human Plasma/Serum Using HPLC-ECD

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      Pages 259-276

      Paraoxonases (PON1, PON2, PON3) Analyses In Vitro and In Vivo in Relation to Cardiovascular Diseases

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