Molecular Embryology

Methods and Protocols

Editors:

ISBN: 978-1-58829-431-9 (Print) 978-1-60327-483-8 (Online)

Table of contents (54 protocols)

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  1. Front Matter

    Pages i-xvii

  2. The Mouse Embryo

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      Pages 3-5

      The Mouse as a Developmental Model

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      Pages 7-22

      Culture of Postimplantation Mouse Embryos

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      Pages 23-30

      Organ Culture in the Analysis of Tissue Interactions

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      Pages 31-36

      Treatment of Mice with Retinoids In Vivo and In Vitro

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      Pages 37-45

      Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage

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      Pages 47-70

      Cell Grafting and Labeling in Postimplantation Mouse Embryos

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      Pages 71-109

      Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs

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      Pages 111-132

      Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells

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      Pages 133-148

      Gene Trapping in Mouse Embryonic Stem Cells

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      Pages 149-164

      Application of lacZ Transgenic Mice to Cell Lineage Studies

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      Pages 165-186

      Transgenic RNA Interference to Investigate Gene Function in the Mouse

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      Pages 187-199

      Mouse Primordial Germ Cells

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      Pages 201-220

      Gene Transfer to the Rodent Embryo by Retroviral Vectors

  3. The Chicken Embryo

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      Pages 223-230

      The Avian Embryo

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      Pages 231-234

      Chick Embryos

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      Pages 235-253

      New Culture

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      Pages 255-264

      EC Culture

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      Pages 265-276

      Grafting Hensen's Node

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      Pages 277-287

      Grafting of Somites

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      Pages 289-303

      Microsurgical Manipulation of the Notochord

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