Drug-DNA Interaction Protocols

Editors:

ISBN: 978-1-60327-417-3 (Print) 978-1-60327-418-0 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-23

    Quantitative Analysis of Small Molecule–Nucleic Acid Interactions with a Biosensor Surface and Surface Plasmon Resonance Detection

  3. No Access

    Book Protocol

    Pages 25-35

    Thermal Melting Studies of Ligand DNA Interactions

  4. No Access

    Book Protocol

    Pages 37-54

    Circular and Linear Dichroism of Drug-DNA Systems

  5. No Access

    Book Protocol

    Pages 55-70

    Drug Binding to DNA⋅RNA Hybrid Structures

  6. No Access

    Book Protocol

    Pages 71-88

    Quantification of Binding Data Using Capillary Electrophoresis

  7. No Access

    Book Protocol

    Pages 89-101

    Determination of Equilibrium Association Constants of Ligand–DNA Complexes by Electrospray Mass Spectrometry

  8. No Access

    Book Protocol

    Pages 103-118

    Detection of Adriamycin-DNA Adducts by Accelerator Mass Spectrometry

  9. No Access

    Book Protocol

    Pages 119-131

    Molecular Modelling Methods to Quantitate Drug-DNA Interactions

  10. No Access

    Book Protocol

    Pages 133-152

    Application of Anomalous Diffraction Methods to the Study of DNA and DNA-Complexes

  11. No Access

    Book Protocol

    Pages 153-172

    DNase I Footprinting

  12. No Access

    Book Protocol

    Pages 173-192

    Methods to Characterize the Effect of DNA-Modifying Compounds on Nucleosomal DNA

  13. No Access

    Book Protocol

    Pages 193-205

    REPSA: Combinatorial Approach for Identifying Preferred Drug-DNA Binding Sequences

  14. No Access

    Book Protocol

    Pages 207-222

    In vitro Transcription Assay for Resolution of Drug-DNA Interactions at Defined DNA Sequences

  15. No Access

    Book Protocol

    Pages 223-233

    In vitro Footprinting of Promoter Regions Within Supercoiled Plasmid DNA

  16. No Access

    Book Protocol

    Pages 235-256

    Topoisomerase I-Mediated DNA Relaxation as a Tool to Study Intercalation of Small Molecules into Supercoiled DNA

  17. No Access

    Book Protocol

    Pages 257-266

    A High-Throughput Assay for DNA Topoisomerases and Other Enzymes, Based on DNA Triplex Formation

  18. No Access

    Book Protocol

    Pages 267-282

    Measurement of DNA Interstrand Crosslinking in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay

  19. No Access

    Book Protocol

    Pages 283-302

    Measurement of DNA Interstrand Crosslinking in Naked DNA Using Gel-Based Methods

  20. No Access

    Book Protocol

    Pages 303-313

    An Evaluation Cascade for G-Quadruplex Telomere Targeting Agents in Human Cancer Cells

  21. Back Matter

    Pages 315-318