DNA Damage Detection In Situ, Ex Vivo, and In Vivo

Methods and Protocols

Editors:

ISBN: 978-1-60327-408-1 (Print) 978-1-60327-409-8 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xiii

  2. Detection in Tissue Sections

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-13

      In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques

    3. No Access

      Book Protocol

      Pages 15-27

      Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage

    4. No Access

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      Pages 29-36

      EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level

    5. No Access

      Book Protocol

      Pages 37-48

      In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase

    6. No Access

      Book Protocol

      Pages 49-63

      In Situ Ligation: A Decade and a Half of Experience

    7. No Access

      Book Protocol

      Pages 65-75

      In Situ Ligation Simplified: Using PCR Fragments for Detection of Double-Strand DNA Breaks in Tissue Sections

    8. No Access

      Book Protocol

      Pages 77-87

      5′OH DNA Breaks in Apoptosis and Their Labeling by Topoisomerase-Based Approach

  3. Detection in Cell Cultures

    1. Front Matter

      Pages 89-89

    2. No Access

      Book Protocol

      Pages 91-101

      Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry

    3. No Access

      Book Protocol

      Pages 103-114

      Fluorochrome-Labeled Inhibitors of Caspases: Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis

    4. No Access

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      Pages 115-132

      Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair

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      Pages 133-147

      Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH)

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      Pages 149-161

      Co-localization of DNA Repair Proteins with UV-Induced DNA Damage in Locally Irradiated Cells

  4. Detection in Live Tissues, Blood, Urine, Sperm

    1. Front Matter

      Pages 163-163

    2. No Access

      Book Protocol

      Pages 165-187

      Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects at High and Low Frequencies

    3. No Access

      Book Protocol

      Pages 189-205

      Quantifying Etheno–DNA Adducts in Human Tissues, White Blood Cells, and Urine by Ultrasensitive 32P-Postlabeling and Immunohistochemistry

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      Book Protocol

      Pages 207-215

      ELISpot Assay as a Tool to Study Oxidative Stress in Peripheral Blood Mononuclear Cells

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      Book Protocol

      Pages 217-234

      Cytokinesis-Block Micronucleus Cytome Assay in Lymphocytes

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      Pages 235-248

      Buccal Micronucleus Cytome Assay

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      Pages 249-270

      γ-H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts, and Skin

    8. No Access

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      Pages 271-278

      Immunologic Detection of Benzo(a)pyrene–DNA Adducts

    9. No Access

      Book Protocol

      Pages 279-289

      Non-invasive Assessment of Oxidatively Damaged DNA: Liquid Chromatography-Tandem Mass Spectrometry Analysis of Urinary 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine

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