Volume 1027 2013

Lipoproteins and Cardiovascular Disease

Methods and Protocols

Editors:

ISBN: 978-1-60327-368-8 (Print) 978-1-60327-369-5 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-x

  2. RNA and Gene Expression

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-17

      Cloning Full-Length Transcripts and Transcript Variants Using 5′ and 3′ RACE

    3. No Access

      Book Protocol

      Pages 19-45

      Monitoring Gene Expression: Quantitative Real-Time RT-PCR

    4. No Access

      Book Protocol

      Pages 47-84

      Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases

    5. No Access

      Book Protocol

      Pages 85-121

      Northern Analysis of Gene Expression

    6. No Access

      Book Protocol

      Pages 123-135

      Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

  3. Sequencing

    1. Front Matter

      Pages 137-137

    2. No Access

      Book Protocol

      Pages 139-155

      Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research

    3. No Access

      Book Protocol

      Pages 157-179

      Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research

  4. Transgenic, Knockout, and Knockdown Methodologies

    1. Front Matter

      Pages 181-181

    2. No Access

      Book Protocol

      Pages 183-201

      Strategies for Designing Transgenic DNA Constructs

    3. No Access

      Book Protocol

      Pages 203-215

      Purification of Plasmid and BAC Transgenic DNA Constructs

    4. No Access

      Book Protocol

      Pages 217-232

      Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production

    5. No Access

      Book Protocol

      Pages 233-251

      Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes

    6. No Access

      Book Protocol

      Pages 253-271

      Generation of General and Tissue-Specific Gene Knockout Mouse Models

    7. No Access

      Book Protocol

      Pages 273-307

      Adeno-associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism

    8. No Access

      Book Protocol

      Pages 309-324

      Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology

  5. Special Topics

    1. Front Matter

      Pages 325-325

    2. No Access

      Book Protocol

      Pages 327-342

      Chromatin Immunoprecipitation

    3. No Access

      Book Protocol

      Pages 343-352

      Measurement of Lecithin–Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate

    4. No Access

      Book Protocol

      Pages 353-367

      Native–Native 2D Gel Electrophoresis for HDL Subpopulation Analysis

    5. No Access

      Book Protocol

      Pages 369-385

      Western Blots

  6. Back Matter

    Pages 387-391