Clostridium difficile

Methods and Protocols

Editors:

ISBN: 978-1-60327-364-0 (Print) 978-1-60327-365-7 (Online)

Table of contents (15 protocols)

  1. Front Matter

    Pages i-x

  2. Introduction to C. difficile and the Disease It Causes

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-7

      Clostridium difficile: No Longer an Enigmatic Pathogen?

    3. No Access

      Book Protocol

      Pages 9-35

      Clostridium difficile and the Disease It Causes

  3. Isolation and Culture Techniques

    1. Front Matter

      Pages 37-37

    2. No Access

      Book Protocol

      Pages 39-52

      Clostridium difficile Isolation and Culture Techniques

  4. Methods for Typing and Epidemiological Studies

    1. Front Matter

      Pages 53-53

    2. No Access

      Book Protocol

      Pages 55-65

      Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping

    3. No Access

      Book Protocol

      Pages 67-76

      Clostridium difficile Toxinotyping

    4. No Access

      Book Protocol

      Pages 77-90

      Multilocus Sequence Typing for Clostridium difficile

  5. Biochemistry of the Organism

    1. Front Matter

      Pages 91-91

    2. No Access

      Book Protocol

      Pages 93-115

      Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes

    3. No Access

      Book Protocol

      Pages 117-134

      Dissecting the Cell Surface

    4. No Access

      Book Protocol

      Pages 135-146

      Human Intestinal Epithelial Response(s) to Clostridium difficile

  6. Genomics

    1. Front Matter

      Pages 147-147

    2. No Access

      Book Protocol

      Pages 149-162

      Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays

  7. Development of Systems for Genetic Analysis of the Organism

    1. Front Matter

      Pages 163-163

    2. No Access

      Book Protocol

      Pages 165-182

      ClosTron-Targeted Mutagenesis

    3. No Access

      Book Protocol

      Pages 183-201

      Methods for Gene Cloning and Targeted Mutagenesis

    4. No Access

      Book Protocol

      Pages 203-211

      Transposon Mutagenesis in Clostridium difficile

  8. Animal Models of Disease

    1. Front Matter

      Pages 213-213

    2. No Access

      Book Protocol

      Pages 215-227

      Refinement of the Hamster Model of Clostridium difficile Disease

    3. No Access

      Book Protocol

      Pages 229-237

      Methods for Working with the Mouse Model

  9. Back Matter

    Pages 239-241