PCR Detection of Microbial Pathogens

Editors:

ISBN: 978-1-60327-352-7 (Print) 978-1-60327-353-4 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-16

    Real-Time Quantitative PCR, Pathogen Detection and MIQE

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    Pages 17-48

    Overcoming Inhibition in Real-Time Diagnostic PCR

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    Pages 49-79

    Quality in the Molecular Microbiology Laboratory

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    Pages 81-90

    Pre-analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood

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    Pages 91-103

    Detection of Bacterial Contamination in Platelet Concentrates Using Flow Cytometry and Real-Time PCR Methods

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    Pages 105-113

    Multiplex Real-Time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton–Valentine Leukocidin from Clinical Samples

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    Pages 115-123

    PCR Detection of Haemophilus influenzae from Respiratory Specimens

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    Pages 125-133

    Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-Time PCR

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    Pages 135-147

    Utilization of Multiple Real-Time PCR Assays for the Diagnosis of Bordetella spp. in Clinical Specimens

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    Pages 149-158

    Detection of Mycoplasma pneumoniae by Real-Time PCR

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    Pages 159-170

    Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia

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    Pages 171-199

    Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens

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    Pages 201-216

    Direct Detection of Mycobacterium ulcerans in Clinical Specimens and Environmental Samples

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    Pages 217-228

    Detection of Bartonella spp. DNA in Clinical Specimens Using an Internally Controlled Real-Time PCR Assay

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    Pages 229-245

    Simultaneous Direct Identification of Genital Microorganisms in Voided Urine Using Multiplex PCR-Based Reverse Line Blot Assays

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    Pages 247-256

    Diagnosis of Clostridium difficile Infection Using Real-Time PCR

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    Pages 257-266

    Detection of Pathogenic Leptospira spp. Through Real-Time PCR (qPCR) Targeting the LipL32 Gene

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    Pages 267-277

    Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli Using Loop-Mediated Isothermal Amplification

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    Pages 279-287

    PCR Detection of Helicobacter pylori in Clinical Samples

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    Pages 289-305

    Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-Time PCR

  22. Back Matter

    Pages 315-317

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