Cell-Free Protein Production

Methods and Protocols

Editors:

ISBN: 978-1-60327-330-5 (Print) 978-1-60327-331-2 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-10

    Cell-Free Protein Preparation Through Prokaryotic Transcription–Translation Methods

  3. No Access

    Book Protocol

    Pages 11-21

    PURE Technology

  4. No Access

    Book Protocol

    Pages 23-30

    The Cell-Free Protein Synthesis System from Wheat Germ

  5. No Access

    Book Protocol

    Pages 31-42

    A Cell-Free Protein Synthesis System from Insect Cells

  6. No Access

    Book Protocol

    Pages 43-52

    Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells

  7. No Access

    Book Protocol

    Pages 53-62

    Analysis of Protein Functions Through a Bacterial Cell-Free Protein Expression System

  8. No Access

    Book Protocol

    Pages 63-72

    Cell-Free-Based Protein Microarray Technology Using Agarose/DNA Microplate

  9. No Access

    Book Protocol

    Pages 73-83

    An Efficient Approach to the Production of Vaccines Against the Malaria Parasite

  10. No Access

    Book Protocol

    Pages 85-99

    Protein Engineering Accelerated by Cell-Free Technology

  11. No Access

    Book Protocol

    Pages 101-111

    Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds

  12. No Access

    Book Protocol

    Pages 113-126

    NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System

  13. No Access

    Book Protocol

    Pages 127-147

    Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination

  14. No Access

    Book Protocol

    Pages 149-160

    Cell-Free Protein Synthesis for Structure Determination by X-ray Crystallography

  15. No Access

    Book Protocol

    Pages 161-171

    Production of Multi-Subunit Complexes on Liposome Through an E. coli Cell-Free Expression System

  16. No Access

    Book Protocol

    Pages 173-185

    Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System

  17. No Access

    Book Protocol

    Pages 187-212

    Strategies for the Cell-Free Expression of Membrane Proteins

  18. No Access

    Book Protocol

    Pages 213-218

    Production of Membrane Proteins Through the Wheat-Germ Cell-Free Technology

  19. No Access

    Book Protocol

    Pages 219-225

    Ribosome Display with the PURE Technology

  20. No Access

    Book Protocol

    Pages 227-242

    Incorporation of 3-Azidotyrosine into Proteins Through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification

  21. No Access

    Book Protocol

    Pages 243-256

    Synthesis of Functional Proteins Within Liposomes

  22. Back Matter

    Pages 257-261