Retinoids

Methods and Protocols

Editors:

ISBN: 978-1-60327-324-4 (Print) 978-1-60327-325-1 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Book Protocol

    Pages 1-54

    Quantification of Endogenous Retinoids

  3. No Access

    Book Protocol

    Pages 55-73

    Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids

  4. No Access

    Book Protocol

    Pages 75-83

    Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol

  5. No Access

    Book Protocol

    Pages 85-94

    In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse Agonists

  6. No Access

    Book Protocol

    Pages 95-114

    Physiological Studies of the Interaction Between Opsin and Chromophore in Rod and Cone Visual Pigments

  7. No Access

    Book Protocol

    Pages 115-127

    Measurement of the Mobility of All-Trans-Retinol with Two-Photon Fluorescence Recovery After Photobleaching

  8. No Access

    Book Protocol

    Pages 129-147

    Microfluorometric Measurement of the Formation of All-Trans-Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors

  9. No Access

    Book Protocol

    Pages 149-162

    HPLC / MSN Analysis of Retinoids

  10. No Access

    Book Protocol

    Pages 163-176

    Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular Degeneration

  11. No Access

    Book Protocol

    Pages 177-187

    Fluorescence-Based Technique for Analyzing Retinoic Acid

  12. No Access

    Book Protocol

    Pages 189-207

    The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotropy

  13. No Access

    Book Protocol

    Pages 209-227

    Assay of Retinol-Binding Protein–Transthyretin Interaction and Techniques to Identify Competing Ligands

  14. No Access

    Book Protocol

    Pages 229-245

    Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle

  15. No Access

    Book Protocol

    Pages 247-261

    Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy

  16. No Access

    Book Protocol

    Pages 263-275

    Reverse-Phase High-Performance Liquid Chromatography (HPLC) Analysis of Retinol and Retinyl Esters in Mouse Serum and Tissues

  17. No Access

    Book Protocol

    Pages 277-294

    Detection of Retinoic Acid Catabolism with Reporter Systems and by In Situ Hybridization for CYP26 Enzymes

  18. No Access

    Book Protocol

    Pages 295-313

    Diet in Vitamin A Research

  19. No Access

    Book Protocol

    Pages 315-327

    Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium

  20. No Access

    Book Protocol

    Pages 329-339

    Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina

  21. No Access

    Book Protocol

    Pages 341-361

    Techniques to Study Specific Cell-Surface Receptor-Mediated Cellular Vitamin A Uptake

  22. Back Matter

    Pages 363-365