1996

The Protein Protocols Handbook

Editors:

ISBN: 978-0-89603-338-2 (Print) 978-1-60327-259-9 (Online)

Table of contents (144 protocols)

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  1. Front Matter

    Pages i-xviii

  2. Quantitation of Proteins

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-6

      Protein Determination by UV Absorption

    3. No Access

      Book Protocol

      Pages 7-9

      The Lowry Method for Protein Quantitation

    4. No Access

      Book Protocol

      Pages 11-14

      The Bicinchoninic Acid (BCA) Assay for Protein Quantitation

    5. No Access

      Book Protocol

      Pages 15-20

      The Bradford Method for Protein Quantitation

    6. No Access

      Book Protocol

      Pages 21-28

      Ultrafast Protein Determinations Using Microwave Enhancement

    7. No Access

      Book Protocol

      Pages 29-31

      Quantitation of Tryptophan in Proteins

    8. No Access

      Book Protocol

      Pages 33-38

      Protein Quantitation Using Flow Cytometry

    9. No Access

      Book Protocol

      Pages 39-43

      Copper Iodide Staining of Proteins on Solid Phases

    10. No Access

      Book Protocol

      Pages 45-47

      Kinetic Silver Staining of Proteins Adsorbed to Microtiter Plates

  3. Electrophoresis of Proteins and Peptides and Detection in Gels

    1. Front Matter

      Pages 49-49

    2. No Access

      Book Protocol

      Pages 51-54

      Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins

    3. No Access

      Book Protocol

      Pages 55-61

      SDS Polyacrylamide Gel Electrophoresis of Proteins

    4. No Access

      Book Protocol

      Pages 63-66

      Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins

    5. No Access

      Book Protocol

      Pages 67-81

      Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins

    6. No Access

      Book Protocol

      Pages 83-90

      Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins

    7. No Access

      Book Protocol

      Pages 91-100

      Acid-Urea-Triton Polyacrylamide Gels for Histones

    8. No Access

      Book Protocol

      Pages 101-107

      SDS-Polyacrylamide Gel Electrophoresis of Peptides

    9. No Access

      Book Protocol

      Pages 109-113

      Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels

    10. No Access

      Book Protocol

      Pages 115-119

      Isoelectric Focusing Under Denaturing Conditions

    11. No Access

      Book Protocol

      Pages 121-126

      Radioisotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis

    12. No Access

      Book Protocol

      Pages 127-132

      Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension

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