Molecular Profiling

Methods and Protocols

Editors:

ISBN: 978-1-60327-215-5 (Print) 978-1-60327-216-2 (Online)

Table of contents (28 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-18

    Tumor Staging and Grading: A Primer

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    Pages 19-34

    Clinical Trial Design in the Age of Molecular Profiling

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    Pages 35-48

    Personalized Medicine: Ethics for Clinical Trials

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    Pages 49-57

    Reduction of Preanalytical Variability in Specimen Procurement for Molecular Profiling

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    Pages 59-77

    The Human Side of Cancer Biobanking

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    Pages 79-88

    Introduction to Genomics

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    Pages 89-105

    Genomic Profiling: cDNA Arrays and Oligoarrays

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    Pages 107-118

    Genome-Wide Methylation Profiling in Archival Formalin-Fixed Paraffin-Embedded Tissue Samples

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    Pages 119-138

    An Overview of MicroRNA Methods: Expression Profiling and Target Identification

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    Pages 139-155

    Antibody Validation by Western Blotting

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    Pages 157-178

    Laser Capture Microdissection: ArcturusXT Infrared Capture and UV Cutting Methods

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    Pages 179-200

    Antibody Microarrays: Analysis of Cystic Fibrosis

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    Pages 201-214

    Tissue Microarrays as a Tool in the Discovery and Validation of Predictive Biomarkers

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    Pages 215-235

    Reverse-Phase Protein Microarrays

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    Pages 237-249

    Serum Low-Molecular-Weight Protein Fractionation for Biomarker Discovery

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    Pages 251-264

    Mass Spectrometry-Based Biomarker Discovery

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    Pages 265-277

    Mitochondrial Proteome: Toward the Detection and Profiling of Disease Associated Alterations

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    Pages 279-293

    Adult Neural Stem Cells: Isolation and Propagation

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    Pages 295-309

    Evanescent-Wave Field Imaging: An Introduction to Total Internal Reflection Fluorescence Microscopy

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    Pages 311-324

    Construction and Hyperspectral Imaging of Quantum Dot Lysate Arrays

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