Germline Stem Cells

Editors:

ISBN: 978-1-60327-213-1 (Print) 978-1-60327-214-8 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii

  2. Identification and Regulation of Germline Stem Cells in Model Organisms

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      Book Protocol

      Pages 3-26

      The Development of Germline Stem Cells in Drosophila

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      Pages 27-44

      Analysis of the C. elegans Germline Stem Cell Region

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      Pages 45-59

      Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell

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      Book Protocol

      Pages 61-70

      Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells

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      Book Protocol

      Pages 71-94

      Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus)

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      Book Protocol

      Pages 95-107

      High-Resolution Light Microscopic Characterization of Spermatogonia

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      Book Protocol

      Pages 109-118

      Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes

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      Pages 119-126

      Epigenetic Control in Male Germ Cells

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      Pages 127-135

      GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro

  3. In Vitro Culture and Applications of Germline Stem Cells

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      Book Protocol

      Pages 139-148

      Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts

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      Pages 149-162

      Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice

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      Pages 163-179

      Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture

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      Pages 181-192

      Deriving Mouse Spermatogonial Stem Cell Lines

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      Pages 193-209

      Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes

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      Book Protocol

      Pages 211-231

      Testicular Germ Cell Tumors in Mice

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      Book Protocol

      Pages 233-265

      Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries

  4. Back Matter

    Pages 673-677