Characterization of Nanoparticles Intended for Drug Delivery

Editors:

ISBN: 978-1-60327-197-4 (Print) 978-1-60327-198-1 (Online)

Table of contents (27 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xii

  2. Introduction and Discussion

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-8

      Unique Benefits of Nanotechnology to Drug Delivery and Diagnostics

    3. No Access

      Book Protocol

      Pages 9-15

      Challenges for Nanoparticle Characterization

    4. No Access

      Book Protocol

      Pages 17-31

      Considerations When Submitting Nanotherapeutics to FDA/CDER for Regulatory Review

  3. Assays to Determine Physical & Chemical Properties

    1. Front Matter

      Pages 33-33

    2. No Access

      Book Protocol

      Pages 35-52

      Measuring the Hydrodynamic Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering

    3. No Access

      Book Protocol

      Pages 53-61

      Characterization of Nanoparticles by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

    4. No Access

      Book Protocol

      Pages 63-70

      Zeta Potential Measurement

    5. No Access

      Book Protocol

      Pages 71-82

      Size Measurement of Nanoparticles Using Atomic Force Microscopy

    6. No Access

      Book Protocol

      Pages 83-91

      Biological Tissue and Cell Culture Specimen Preparation for TEM Nanoparticle Characterization

    7. No Access

      Book Protocol

      Pages 93-99

      SEM X-Ray Microanalysis of Nanoparticles Present in Tissue or Cultured Cell Thin Sections

    8. No Access

      Book Protocol

      Pages 101-108

      Detecting and Measuring Free Gadolinium in Nanoparticles for MRI Imaging

    9. No Access

      Book Protocol

      Pages 109-117

      Lipid Component Quantitation by Thin Layer Chromatography

  4. Assays for Sterility

    1. Front Matter

      Pages 119-119

    2. No Access

      Book Protocol

      Pages 121-130

      Detection and Quantitative Evaluation of Endotoxin Contamination in Nanoparticle Formulations by LAL-Based Assays

    3. No Access

      Book Protocol

      Pages 131-134

      Analysis of Microbial Contamination in Nanoparticle Formulations

  5. Assays for In Vitro Detection and Quantitation

    1. Front Matter

      Pages 135-135

    2. No Access

      Book Protocol

      Pages 137-143

      Gold Nanoparticle Quantitation via Fluorescence in Solution and Cell Culture

    3. No Access

      Book Protocol

      Pages 145-153

      Quantitation of Nanoparticles in Serum Matrix by Capillary Electrophoresis

  6. In Vitro Assays for Toxicity

    1. Front Matter

      Pages 155-155

    2. No Access

      Book Protocol

      Pages 157-165

      Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells

    3. No Access

      Book Protocol

      Pages 167-172

      Monitoring Nanoparticle-Treated Hepatocarcinoma Cells for Apoptosis

    4. No Access

      Book Protocol

      Pages 173-179

      Detecting Reactive Oxygen Species in Primary Hepatocytes Treated with Nanoparticles

    5. No Access

      Book Protocol

      Pages 181-189

      Assay to Detect Lipid Peroxidation upon Exposure to Nanoparticles

    6. No Access

      Book Protocol

      Pages 191-198

      Monitoring Glutathione Homeostasis in Nanoparticle-Treated Hepatocytes

previous Page of 2