Bacteriophages

Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

Editors:

ISBN: 978-1-58829-682-5 (Print) 978-1-60327-164-6 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-xxii

  2. Bacteriophage Isolation

    1. Front Matter

      Pages 1-1

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      Pages 3-14

      Methods for the Isolation of Viruses from Environmental Samples

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      Pages 15-21

      Bacteriophage Enrichment from Water and Soil

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      Pages 23-32

      Isolation of Phage via Induction of Lysogens

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      Pages 33-42

      Isolation of Cyanophages from Aquatic Environments

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      Pages 43-54

      Isolation of Viruses from High Temperature Environments

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      Pages 55-66

      Isolation of Novel Large and Aggregating Bacteriophages

  3. Bacteriophage Characterization

    1. Front Matter

      Pages 67-67

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      Book Protocol

      Pages 69-76

      Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

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      Book Protocol

      Pages 77-80

      Enumeration of Bacteriophages by the Direct Plating Plaque Assay

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      Pages 81-85

      Enumeration of Bacteriophages Using the Small Drop Plaque Assay System

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      Book Protocol

      Pages 87-95

      Determination of Virus Abundance by Epifluorescence Microscopy

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      Pages 97-111

      Enumeration of Bacteriophages Using Flow Cytometry

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      Pages 113-126

      Basic Phage Electron Microscopy

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      Pages 127-140

      Phage Classification and Characterization

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      Pages 141-149

      Phage Host Range and Efficiency of Plating

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      Pages 151-155

      Measurement of the Rate of Attachment of Bacteriophage to Cells

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      Pages 157-160

      Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors

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      Pages 161-174

      Bacteriophage Plaques: Theory and Analysis

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      Pages 175-202

      Practical Methods for Determining Phage Growth Parameters

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      Pages 203-219

      Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes

  4. Bacteriophage-Host Interactions

    1. Front Matter

      Pages 220-220

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      Book Protocol

      Pages 223-237

      Construction of Phage Mutants

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