Structural Proteomics

High-Throughput Methods

Editors:

ISBN: 978-1-58829-809-6 (Print) 978-1-60327-058-8 (Online)

Table of contents (39 protocols)

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  1. Front Matter

    Pages i-xxvi

  2. Protein Target Selection, Bioinformatic Approaches, and Data Management

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      Pages 3-25

      Target Selection for Structural Genomics: An Overview

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      Pages 27-35

      A General Target Selection Method for Crystallographic Proteomics

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      Pages 37-47

      Target Selection: Triage in the Structural Genomics Battlefield

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      Pages 49-79

      Data Management in Structural Genomics: An Overview

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      Pages 81-101

      Data Deposition and Annotation at the Worldwide Protein Data Bank

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      Pages 103-115

      Prediction of Protein Disorder

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      Pages 117-143

      Protein Domain Prediction

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      Pages 145-159

      Protein Structure Modeling with MODELLER

  3. Protein Production

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      Pages 163-173

      High Throughput Cloning with Restriction Enzymes

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      Pages 175-186

      Automated Recombinant Protein Expression Screening in Escherichia coli

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      Pages 187-195

      From No Expression to High-Level Soluble Expression in Escherichia coli by Screening a Library of the Target Proteins with Randomized N-Termini

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      Pages 197-208

      Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli

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      Pages 209-220

      A High Throughput Platform for Eukaryotic Genes

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      Pages 221-246

      High Throughput Production of Recombinant Human Proteins for Crystallography

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      Pages 247-256

      Assembly of Protein Complexes by Coexpression in Prokaryotic and Eukaryotic Hosts: an Overview

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      Pages 257-268

      Cell-Free Protein Synthesis for Analysis by NMR Spectroscopy

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      Pages 269-275

      A Medium or High Throughput Protein Refolding Assay

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      Pages 277-295

      Structural Proteomics of Membrane Proteins: a Survey of Published Techniques and Design of a Rational High Throughput Strategy

  4. Biophysical and Functional Characterization of Proteins

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      Pages 299-318

      Methods for Protein Characterization by Mass Spectrometry, Thermal Shift (ThermoFluor) Assay, and Multiangle or Static Light Scattering

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      Pages 319-330

      High Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram ScaleHigh Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram Scale

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