Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

Editors:

ISBN: 978-1-58829-700-6 (Print) 978-1-60327-040-3 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii

  2. Introduction

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-19

      Basic Principles of Fluorescence and Energy Transfer

  3. Applications

    1. Front Matter

      Pages 21-21

    2. No Access

      Book Protocol

      Pages 23-31

      Multiplex Detection of Mutations

    3. No Access

      Book Protocol

      Pages 33-43

      Detection of Salmonella Species in Foodstuffs

    4. No Access

      Book Protocol

      Pages 45-58

      Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR

    5. No Access

      Book Protocol

      Pages 59-72

      Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma

    6. No Access

      Book Protocol

      Pages 73-87

      Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-time PCR and RT-PCR

    7. No Access

      Book Protocol

      Pages 89-98

      High-Throughput Real-Time PCR

    8. No Access

      Book Protocol

      Pages 99-115

      Design and Use of Scorpions Fluorescent Signaling Molecules

    9. No Access

      Book Protocol

      Pages 117-133

      Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification

  4. New Technologies

    1. Front Matter

      Pages 135-135

    2. No Access

      Book Protocol

      Pages 137-160

      EasyBeacons™ for the Detection of Methylation Status of Single CpG Duplets

    3. No Access

      Book Protocol

      Pages 161-170

      Quenched Autoligation Probes

    4. No Access

      Book Protocol

      Pages 171-185

      HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination

    5. No Access

      Book Protocol

      Pages 187-197

      FIT-Probes in Real-Time PCR

    6. No Access

      Book Protocol

      Pages 199-206

      SNP Genotyping by Unlabeled Probe Melting Analysis

  5. Non-Nucleic Acid Targets

    1. Front Matter

      Pages 207-207

    2. No Access

      Book Protocol

      Pages 209-224

      Molecular Beacons for Protein—DNA Interaction Studies

    3. No Access

      Book Protocol

      Pages 225-235

      DNA Polymerase Profiling

    4. No Access

      Book Protocol

      Pages 237-250

      Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions

    5. No Access

      Book Protocol

      Pages 251-263

      Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes

  6. Back Matter

    Pages 265-266