Human Embryogenesis

Methods and Protocols

Editors:

ISBN: 978-1-60327-008-3 (Print) 978-1-60327-009-0 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-x

  2. Introduction: What is Human Embryogenesis?

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-7

      Human Embryogenesis: Overview

  3. Studying Embryo Implantation: From Trophoblasts to Embryo

    1. Front Matter

      Pages 9-9

    2. No Access

      Book Protocol

      Pages 11-61

      Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation

    3. No Access

      Book Protocol

      Pages 63-72

      Coculture of Decidua and Trophoblast to Study Proliferation and Invasion

    4. No Access

      Book Protocol

      Pages 73-87

      Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells’ Calcium Uptake

    5. No Access

      Book Protocol

      Pages 89-102

      Endocrinology and Cell Signaling in Human Villous Trophoblast

    6. No Access

      Book Protocol

      Pages 103-123

      Gestation-Induced Uterine Vascular Remodeling

  4. Steriodogenesis and Reproductive System

    1. Front Matter

      Pages 125-125

    2. No Access

      Book Protocol

      Pages 127-136

      Placental and Fetal Steroidogenesis

    3. No Access

      Book Protocol

      Pages 137-157

      Current Methods in Investigating the Development of the Female Reproductive System

    4. No Access

      Book Protocol

      Pages 159-167

      A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins

    5. No Access

      Book Protocol

      Pages 169-177

      A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis

  5. From Embryo to Fetal Development

    1. Front Matter

      Pages 179-179

    2. No Access

      Book Protocol

      Pages 181-194

      Genetic Dissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow Cytometry

    3. No Access

      Book Protocol

      Pages 195-203

      Immune System: Maturation of Myeloid Cells

    4. No Access

      Book Protocol

      Pages 205-224

      Functional Development of Human Fetal Gastrointestinal Tract

    5. No Access

      Book Protocol

      Pages 225-237

      Renal and Cardiac Na+–K+-ATPase and Aconitase in a Rat Model of Fetal Programming

  6. Embryotoxicity, in Vitro Fertilization, Cloning, and Ethics

    1. Front Matter

      Pages 239-239

    2. No Access

      Book Protocol

      Pages 241-249

      Assessment of Embryotoxicity Using Mouse Embryo Culture

    3. No Access

      Book Protocol

      Pages 251-266

      Fertilization In Vitro

    4. No Access

      Book Protocol

      Pages 267-279

      Animal Cloning by Somatic Cell Nuclear Transfer

    5. No Access

      Book Protocol

      Pages 281-305

      The Human Embryo: Ethical and Legal Aspects

  7. Back Matter

    Pages 307-312