Affinity Chromatography

Methods and Protocols

Editors:

ISBN: 978-1-58829-659-7 (Print) 978-1-59745-582-4 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xii

  2. Affinity Chromatography

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 1-23

      Affinity Chromatography

  3. Various Modes of Affinity Chromatography

    1. Front Matter

      Pages 23-23

    2. No Access

      Book Protocol

      Pages 25-36

      Immobilized Metal Ion Affinity Chromatography of Native Proteins

    3. No Access

      Book Protocol

      Pages 37-52

      Affinity Precipitation of Proteins Using Metal Chelates

    4. No Access

      Book Protocol

      Pages 53-60

      Immunoaffinity Chromatography

    5. No Access

      Book Protocol

      Pages 61-70

      Dye Ligand Chromatography

    6. No Access

      Book Protocol

      Pages 71-90

      Purification of Proteins Using Displacement Chromatography

  4. Affinity Chromatography Using Purification Tags

    1. Front Matter

      Pages 91-91

    2. No Access

      Book Protocol

      Pages 93-110

      Rationally Designed Ligands for Use in Affinity Chromatography

    3. No Access

      Book Protocol

      Pages 111-124

      Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography

    4. No Access

      Book Protocol

      Pages 125-136

      Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein

    5. No Access

      Book Protocol

      Pages 137-150

      Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins

    6. No Access

      Book Protocol

      Pages 151-168

      Methods for the Purification of HQ-Tagged Proteins

    7. No Access

      Book Protocol

      Pages 169-190

      Amylose Affinity Chromatography of Maltose-Binding Protein

    8. No Access

      Book Protocol

      Pages 191-210

      Methods for Detection of Protein–Proteinnl and Protein–DNA Interactions Using HaloTag ™

    9. No Access

      Book Protocol

      Pages 211-228

      Site-Specific Cleavage of Fusion Proteins

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      Book Protocol

      Pages 229-243

      The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags

  5. Various Applications of Affinity Chromatography

    1. Front Matter

      Pages 245-245

    2. No Access

      Book Protocol

      Pages 247-256

      Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels

    3. No Access

      Book Protocol

      Pages 257-274

      Monolithic Bioreactors for Macromolecules

    4. No Access

      Book Protocol

      Pages 275-284

      Plasmid DNA Purification Via the Use of a Dual Affinity Protein

    5. No Access

      Book Protocol

      Pages 285-294

      Affinity Chromatography of Phosphorylated Proteins

    6. No Access

      Book Protocol

      Pages 295-302

      Protein Separation Using Immobilized Phospholipid Chromatography

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