Avidin-Biotin Interactions

Methods and Applications

Editors:

ISBN: 978-1-58829-583-5 (Print) 978-1-59745-579-4 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii

  2. No Access

    Book Protocol

    Pages 1-12

    Preparation of Avidin Conjugates

  3. No Access

    Book Protocol

    Pages 13-23

    Coupling of Antibodies With Biotin

  4. No Access

    Book Protocol

    Pages 25-34

    Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin

  5. No Access

    Book Protocol

    Pages 35-50

    Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation

  6. No Access

    Book Protocol

    Pages 51-61

    Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis

  7. No Access

    Book Protocol

    Pages 63-71

    Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS–PAGE Using Biotin-4-Fluorescein

  8. No Access

    Book Protocol

    Pages 73-88

    Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives

  9. No Access

    Book Protocol

    Pages 89-100

    Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins

  10. No Access

    Book Protocol

    Pages 101-110

    High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli

  11. No Access

    Book Protocol

    Pages 111-128

    Endogenous Biotin in Rat Brain

  12. No Access

    Book Protocol

    Pages 129-138

    Pseudo-Immunolabelling With the Avidin–Biotin–Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina

  13. No Access

    Book Protocol

    Pages 139-148

    Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones

  14. No Access

    Book Protocol

    Pages 149-155

    Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay

  15. No Access

    Book Protocol

    Pages 157-170

    Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin

  16. No Access

    Book Protocol

    Pages 171-185

    Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure

  17. No Access

    Book Protocol

    Pages 187-207

    A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin

  18. No Access

    Book Protocol

    Pages 209-220

    Biotin–Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications

  19. Back Matter

    Pages 221-232