Viral Applications of Green Fluorescent Protein

Methods and Protocols

Editors:

ISBN: 978-1-934115-87-9 (Print) 978-1-59745-559-6 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-11

    Using Fluorescent Proteins to Study Poxvirus Morphogenesis

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    Pages 13-32

    Molecular Imaging of Human Embryonic Stem Cells

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    Pages 33-44

    Use of a GFP-PML-Expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection

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    Pages 45-61

    Dual-Color Imaging of Tumor Angiogenesis

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    Pages 63-95

    Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System

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    Pages 97-106

    Expression of EGFP by Adenovirus-Mediated Gene Transfer in the Central Nervous System

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    Pages 107-123

    Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes

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    Pages 125-135

    Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry

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    Pages 137-150

    Production of Multicistronic HIV-1 Based Lentiviral Vectors

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    Pages 151-163

    Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps

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    Pages 165-175

    Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs

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    Pages 177-197

    GFP-Lentiviral Vectors Targeting for NeuroAIDS

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    Pages 199-213

    Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery

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    Pages 215-226

    Selective Protein Expression Within the CNS Using Hybrid Lentivirus

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    Pages 227-238

    Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein

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    Pages 239-248

    The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication

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    Pages 249-260

    Modified Bovine Herpesvirus 1 for Protein Secretion

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    Pages 261-274

    Identification of Viral Peptide Fragments for Vaccine Development

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    Pages 275-285

    Use of GFP to Investigate Expression of Plant-Derived Vaccines

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    Pages 287-297

    Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions

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