Therapeutic Antibodies

Methods and Protocols

Editors:

ISBN: 978-1-934115-92-3 (Print) 978-1-59745-554-1 (Online)

Table of contents (32 protocols)

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  1. Front Matter

    Pages i-xviii

  2. Recombinant Antigens

    1. Front Matter

      Pages 1-1

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      Book Protocol

      Pages 1-27

      Therapeutic Antibodies: Current State and Future Trends – Is a Paradigm Change Coming Soon?

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      Book Protocol

      Pages 31-58

      Preparation of Recombinant Viral Glycoproteins for Novel and Therapeutic Antibody Discovery

  3. Antibody Libraries

    1. Front Matter

      Pages 1-1

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      Book Protocol

      Pages 61-80

      Design of a Human Synthetic Combinatorial Library of Single-Chain Antibodies

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      Pages 81-99

      Construction of a Human Antibody Domain (VH) Library

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      Pages 101-128

      Generation and Selection of Rabbit Antibody Libraries by Phage Display

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      Pages 129-142

      Construction of a Large Naïve Human Phage-Displayed Fab Library Through One-Step Cloning

  4. Antibody Discovery

    1. Front Matter

      Pages 1-1

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      Pages 145-160

      Identification of Target and Function Specific Antibodies for Effective Drug Delivery

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      Pages 161-174

      Screening of Specific Internalization Fab Fragment from Human Naive Phage Library by Combinational Bio-Panning

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      Pages 175-186

      Competitive Antigen Panning for Selection of HIV-1 Neutralizing Human Monoclonal Antibodies Specific for gp41

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      Pages 187-216

      Selection of Non-aggregating VH Binders from Synthetic VH Phage-Display Libraries

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      Pages 217-239

      Isolation of Full-Length IgG Antibodies from Combinatorial Libraries Expressed in Escherichia coli

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      Pages 241-260

      Multiplexed Flow Cytometry: High-Throughput Screening of Single-Chain Antibodies

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      Pages 261-277

      Human Antibody Repertoires

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      Pages 279-289

      Rapid Screening Platform for Stabilization of scFvs in Escherichia coli

  5. Antibody Engineering

    1. Front Matter

      Pages 1-1

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      Book Protocol

      Pages 293-308

      In Vitro Antibody Affinity Maturation Targeting Germline Hotspots

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      Pages 309-322

      Affinity Maturation by Phage Display

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      Pages 323-336

      Production of Chimeric Heavy-Chain Antibodies

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      Pages 337-352

      Mammalian Cell Display for Antibody Engineering

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      Pages 353-376

      Improving Antibody Binding Affinity and Specificity for Therapeutic Development

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      Pages 377-404

      Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy

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