Micro and Nano Technologies in Bioanalysis

Methods and Protocols

Editors:

ISBN: 978-1-934115-40-4 (Print) 978-1-59745-483-4 (Online)

Table of contents (41 protocols)

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  1. Front Matter

    Pages i-xix

  2. Applications of microfluidics and nanopores in separation, detection, manipulation, and analysis of biomolecules

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-15

      HPLC-Chip/MS Technology in Proteomic Profiling

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      Pages 17-27

      Nanofluidic Channel Fabrication and Manipulation of DNA Molecules

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      Pages 29-42

      A Single-Molecule Barcoding System using Nanoslits for DNA Analysis

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      Pages 43-52

      Microfluidic Devices with Photodefinable Pseudo-valves for Protein Separation

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      Pages 53-67

      Microfluidic Chips Designed for Measuring Biomolecules Through a Microbead-Based Quantum Dot Fluorescence Assay

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      Pages 69-79

      DNA Focusing Using Microfabricated Electrode Arrays

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      Pages 81-93

      Solid-State Nanopore for Detecting Individual Biopolymers

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      Pages 95-112

      Inserting and Manipulating DNA in a Nanopore with Optical Tweezers

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      Pages 113-127

      Forming an α-Hemolysin Nanopore for Single-Molecule Analysis

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      Pages 129-150

      Nanopore Force Spectroscopy on DNA Duplexes

  3. Technologies of physical science and chemistry in detection and analysis of biomolecules

    1. Front Matter

      Pages 152-152

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      Pages 153-162

      Quantitative Chemical Analysis of Single Cells

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      Pages 163-186

      Trapping and Detection of Single Molecules in Water

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      Pages 187-200

      ZnO Nanorods as an Intracellular Sensor for pH Measurements

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      Pages 201-229

      Analysis of Biomolecules Using Surface Plasmons

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      Pages 231-252

      Use of Residual Dipolar Couplings in Structural Analysis of Protein-Ligand Complexes by Solution NMR Spectroscopy

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      Pages 253-267

      Raman-Assisted X-Ray Crystallography for the Analysis of Biomolecules

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      Pages 269-279

      Methods and Software for Diffuse X-Ray Scattering from Protein Crystals

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      Pages 281-292

      Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis

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      Pages 293-305

      Small-Angle Neutron Scattering for Molecular Biology: Basics and Instrumentation

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      Pages 307-323

      Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-Molecular Complexes

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