Epitope Mapping Protocols

Second Edition

Editors:

ISBN: 978-1-934115-17-6 (Print) 978-1-59745-450-6 (Online)

Table of contents (32 protocols)

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  1. Front Matter

    Pages i-xiii

  2. B-cell Epitope Mapping

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-20

      What Is a B-Cell Epitope?

  3. Whole Antigen Methods

    1. Front Matter

      Pages 22-22

    2. No Access

      Book Protocol

      Pages 23-36

      Structural Basis of Antibody–Antigen Interactions

    3. No Access

      Book Protocol

      Pages 37-57

      Epitope Mapping of Antibody–Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy

    4. No Access

      Book Protocol

      Pages 59-66

      A Solid-Phase Mutual Inhibition Assay with Labeled Antigen

    5. No Access

      Book Protocol

      Pages 67-76

      Epitope Mapping by Surface Plasmon Resonance

    6. No Access

      Book Protocol

      Pages 77-86

      Proteolytic Fragmentation for Epitope Mapping

    7. No Access

      Book Protocol

      Pages 87-101

      Epitope Mapping by Proteolysis of Antigen–Antibody Complexes

    8. No Access

      Book Protocol

      Pages 103-117

      Identifying Residues in Antigenic Determinants by Chemical Modification

    9. No Access

      Book Protocol

      Pages 119-134

      Epitope Mapping by Differential Chemical Modification of Antigens

  4. Peptide Library Approaches

    1. Front Matter

      Pages 136-136

    2. No Access

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      Pages 137-144

      Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides

    3. No Access

      Book Protocol

      Pages 145-167

      Antibody Epitope Mapping Using SPOT™ Peptide Arrays

    4. No Access

      Book Protocol

      Pages 169-180

      Peptide Microarrays for Profiling of Modification State-Specific Antibodies

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      Book Protocol

      Pages 181-201

      Epitope Mapping Using Phage Display Peptide Libraries

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      Book Protocol

      Pages 203-211

      Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries

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      Book Protocol

      Pages 213-223

      Antibody Specificity Profiling on Functional Protein Microarrays

    8. No Access

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      Pages 225-234

      Peptide Microarrays for Determination of Cross-Reactivity

  5. Profiling of Antibody Signatures in Biological Fluids.

    1. Front Matter

      Pages 236-236

    2. No Access

      Book Protocol

      Pages 237-246

      Epitope Mapping Using Randomly Generated Peptide Libraries

    3. No Access

      Book Protocol

      Pages 247-258

      Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease

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      Book Protocol

      Pages 259-272

      Microarrayed Allergen Molecules for Diagnostics of Allergy

    5. No Access

      Book Protocol

      Pages 273-285

      Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays

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