Leukemia

Methods and Protocols

Editors:

ISBN: 978-1-58829-989-5 (Print) 978-1-59745-418-6 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-5

    An Overview: From Discovery of Candidate Mutations to Disease Modeling and Transformation Mechanisms of Acute Leukemia

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    Pages 7-27

    Backtracking of Leukemic Clones to Birth

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    Pages 29-55

    Fluorescence In Situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia

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    Pages 57-70

    Specialized Fluorescence In Situ Hybridization (FISH) Techniques for Leukaemia Research

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    Pages 71-83

    LDI-PCR: Identification of Known and Unknown Gene Fusions of the Human MLL Gene

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    Pages 85-114

    Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences

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    Pages 115-150

    MRD Detection in Acute Lymphoblastic Leukemia Patients Using Ig/TCR Gene Rearrangements as Targets for Real-Time Quantitative PCR

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    Pages 151-177

    Array-Based Comparative Genomic Hybridization as a Tool for Analyzing the Leukemia Genome

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    Pages 179-206

    Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer

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    Pages 207-229

    Retroviral/Lentiviral Transduction and Transformation Assay

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    Pages 231-246

    Gene Expression Profiling of Leukemia Stem Cells

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    Pages 247-262

    Humanized Model to Study Leukemic Stem Cells

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    Pages 263-285

    Model Systems for Examining Effects of Leukemia Associated Oncogenes in Primary Human CD34+ Cells via Retroviral Transduction

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    Pages 287-300

    Ex Vivo Assays to Study Self-Renewal and Long-Term Expansion of Genetically Modified Primary Human Acute Myeloid Leukemia Stem Cells

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    Pages 301-315

    Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse Bone Marrow by Flow Cytometry

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    Pages 317-334

    In Vitro Differentiation of Embryonic Stem Cells as a Model of Early Hematopoietic Development

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    Pages 335-346

    Analysis of the Mouse Placenta as a Hematopoietic Stem Cell Niche

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    Pages 347-367

    Identification of Protein Interaction Partners by the Yeast Two-Hybrid System

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    Pages 369-393

    Complete Array of HOX Gene Expression by RQ-PCR

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    Pages 395-407

    Genome-Wide Determination of DNA Methylation by Hpa II Tiny Fragment Enrichment by Ligation-Mediated PCR (HELP) for the Study of Acute Leukemias

  22. Back Matter

    Pages 425-428

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