Volume 24 2007

Animal Cell Biotechnology

Methods and Protocols

Editors:

ISBN: 978-1-58829-660-3 (Print) 978-1-59745-399-8 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xv

  2. Basic Techniques for Establishing Production Cell Lines

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-21

      Transduction Technologies

    3. No Access

      Book Protocol

      Pages 23-91

      Cells for Gene Therapy and Vector Production

    4. No Access

      Book Protocol

      Pages 93-121

      Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments

    5. No Access

      Book Protocol

      Pages 123-145

      DNA Fingerprinting and Characterization of Animal Cell Lines

  3. Basic Cultivation Techniques

    1. Front Matter

      Pages 147-147

    2. No Access

      Book Protocol

      Pages 149-178

      Microcarrier Cell Culture Technology

    3. No Access

      Book Protocol

      Pages 179-191

      Cell Encapsulation

    4. No Access

      Book Protocol

      Pages 193-202

      Tools for High-Throughput Medium and Process Optimization

  4. Cell Characterization and Analysis

    1. Front Matter

      Pages 203-203

    2. No Access

      Book Protocol

      Pages 205-222

      Cell Counting and Viability Measurements

    3. No Access

      Book Protocol

      Pages 223-237

      Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry

    4. No Access

      Book Protocol

      Pages 239-251

      Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors

    5. No Access

      Book Protocol

      Pages 253-268

      Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture

    6. No Access

      Book Protocol

      Pages 269-284

      Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation

    7. No Access

      Book Protocol

      Pages 285-299

      Measurement of Apoptosis in Cell Culture

    8. No Access

      Book Protocol

      Pages 301-317

      Metabolic Flux Estimation in Mammalian Cell Cultures

  5. Special Cultivation Techniques

    1. Front Matter

      Pages 319-319

    2. No Access

      Book Protocol

      Pages 321-335

      Disposable Bioreactors for Inoculum Production and Protein Expression

    3. No Access

      Book Protocol

      Pages 337-352

      Hollow Fiber Cell Culture

    4. No Access

      Book Protocol

      Pages 353-369

      Cultivation of Mammalian Cells in Fixed-Bed Reactors

    5. No Access

      Book Protocol

      Pages 371-393

      Configuration of Bioreactors

  6. Downstream Techniques

    1. Front Matter

      Pages 395-395

    2. No Access

      Book Protocol

      Pages 397-420

      Membrane Filtration in Animal Cell Culture

    3. No Access

      Book Protocol

      Pages 421-453

      Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins

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