Hepatitis C

Methods and Protocols

Editors:

ISBN: 978-1-58829-970-3 (Print) 978-1-59745-394-3 (Online)

Table of contents (33 protocols)

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  1. Front Matter

    Pages i-xxi

  2. Detection and Quantification of HCV

    1. Front Matter

      Pages 1-1

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      Pages 3-14

      Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays

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      Pages 15-23

      Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen

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      Pages 25-30

      Immunohistochemical Detection of HCV Proteins in Liver Tissue

  3. HCV Nomenclature, Genotyping, and Quasispecies

    1. Front Matter

      Pages 31-31

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      Pages 33-53

      Nomenclature and Numbering of the Hepatitis C Virus

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      Pages 55-71

      A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates

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      Pages 73-81

      Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry

  4. HCV Replication in Vitro

    1. Front Matter

      Pages 83-83

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      Pages 85-94

      Purification and Crystallization of NS5A Domain I of Hepatitis C Virus

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      Pages 95-110

      NS5A Phosphorylation and Hyperphosphorylation

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      Pages 111-124

      Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies

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      Pages 125-143

      Structural and Functional Analysis of the HCV p7 Protein

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      Pages 145-163

      HCV Replicons: Overview and Basic Protocols

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      Pages 165-175

      Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA

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      Pages 177-184

      Studying HCV RNA Synthesis In Vitro with Replication Complexes

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      Pages 185-193

      Proteomics Study of the Hepatitis C Virus Replication Complex

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      Pages 195-209

      Investigation of the Hepatitis C Virus Replication Complex

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      Pages 211-226

      Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease

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      Pages 227-242

      Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay

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      Pages 243-250

      High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System

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      Pages 251-262

      Inhibition of HCV Replication by Small Interfering RNA

  5. HCV Entry and Infection

    1. Front Matter

      Pages 263-263

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      Pages 265-277

      Cellular Receptors and HCV Entry

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